短期给药后小鼠脑内泛醇定位的成像质谱分析。
Imaging mass spectrometry analysis of ubiquinol localization in the mouse brain following short-term administration.
机构信息
Department of Bioscience, Fukui Prefectural University, Eiheiji, Fukui, 910-1195, Japan.
Takasago Analysis Center, Kaneka Techno Research Corporation, 1-8 Miyamaecho Takasagocho, Takasago, Hyogo, 676-8688, Japan.
出版信息
Sci Rep. 2017 Oct 11;7(1):12990. doi: 10.1038/s41598-017-13257-8.
We analyzed the localization of ubiquinol, the reduced form of coenzyme Q10 (Re-CoQ10), in mouse brain sections using matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance imaging mass spectrometry (IMS) to evaluate the effect of dietary Re-CoQ10 in mouse brain. Mice were orally administered Re-CoQ10 for 14 days and brain Re-CoQ10 content was subsequently quantified using liquid chromatography-mass spectrometry. IMS was employed to visualize Re-CoQ10 at a resolution of 150 μm in the mouse brain. Increased Re-CoQ10 was observed in the corpus callosum, hippocampus, midbrain, cerebellum, brain stem, substantia nigra and striatum. These regions are related to movement, memory and vital life functions. Thus, we demonstrated the effect of Re-CoQ10 administration on the specific localization of Re-CoQ10 in the brain.
我们使用基质辅助激光解吸/电离傅里叶变换离子回旋共振成像质谱（IMS）分析了小鼠脑切片中泛醇（辅酶 Q10 的还原形式）的定位，以评估膳食 Re-CoQ10 对小鼠大脑的影响。小鼠经口给予 Re-CoQ1014 天，随后使用液相色谱-质谱法定量测定脑 Re-CoQ10 含量。IMS 用于在 150 μm 的分辨率下可视化小鼠大脑中的 Re-CoQ10。在胼胝体、海马体、中脑、小脑、脑干、黑质和纹状体中观察到 Re-CoQ10 的增加。这些区域与运动、记忆和重要的生命功能有关。因此，我们证明了 Re-CoQ10 给药对 Re-CoQ10 在大脑中特定定位的影响。
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