Montejo de Garcini E, Carrascosa J L, Correas I, Nieto A, Avila J
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
FEBS Lett. 1988 Aug 15;236(1):150-4. doi: 10.1016/0014-5793(88)80304-1.
Tau factor, upon urea treatment, is able to polymerize in vitro. These polymers are composed of tau factor as shown by immunogold staining. The structure of tau polymers is very similar to that of paired helical filaments (PHFs) of Alzheimer's disease in their dimensions as well as in their periodicity. Metal shadowing of both polymers shows a similar twisting. Also, similar peptide maps were found for tau factor and a 33 kDa protein that is the main component of our PHF preparations.
经尿素处理后，Tau因子能够在体外发生聚合。免疫金染色显示，这些聚合物由Tau因子组成。Tau聚合物的结构在尺寸和周期性方面与阿尔茨海默病的双螺旋丝（PHF）非常相似。两种聚合物的金属投影显示出相似的扭曲。此外，还发现Tau因子与一种33 kDa蛋白质（我们的PHF制剂的主要成分）具有相似的肽图谱。
