Meesapyodsuk Dauenpen, Chen Yi, Ye Shengjian, Chapman Robert G, Qiu Xiao
National Research Council Canada, Saskatoon, Saskatchewan, Canada.
Department of Food & Bioproduct Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Metab Eng Commun. 2021 Apr 7;12:e00171. doi: 10.1016/j.mec.2021.e00171. eCollection 2021 Jun.
Docosadienoic acid (DDA, 22:2-13,16) and docosatrienoic acid (DTA, 22:3-13,16,19) are two very long chain polyunsaturated fatty acids (VLCPUFAs) that are recently shown to possess strong anti-inflammatory and antitumor properties. An ELO type elongase (EhELO1) from wild plant can synthesize the two fatty acids by sequential elongation of linoleic acid and alpha-linolenic acid, respectively. Seed-specific expression of this gene in oilseed crop produced a considerable amount of DDA and DTA in transgenic seeds. However, these fatty acids were excluded from the position of triacylglycerols (TAGs). To improve the production level and nutrition value of the VLCPUFAs in the transgenic oilseed crop, a cytoplasmic lysophosphatidic acid acyltransferase (EhLPAAT2) for the incorporation of the two fatty acids into the -2 position of triacylglycerols was identified from . RT-PCR analysis showed that it was preferentially expressed in developing seeds where was exclusively expressed in . Seed specific expression of along with in resulted in the effective incorporation of DDA and DTA at the position of TAGs, thereby increasing the total amount of DDA and DTA in transgenic seeds. To our knowledge, this is the first plant LPAAT that can incorporate VLCPUFAs into TAGs. Improved production of DDA and DTA in the oilseed crop using EhLPAAT2 and EhELO1 provides a real commercial opportunity for high value agriculture products for nutraceutical uses.
二十二碳二烯酸（DDA，22:2-13,16）和二十二碳三烯酸（DTA，22:3-13,16,19）是两种超长链多不饱和脂肪酸（VLCPUFAs），最近研究表明它们具有很强的抗炎和抗肿瘤特性。来自野生植物的一种ELO型延长酶（EhELO1）可以分别通过亚油酸和α-亚麻酸的顺序延长来合成这两种脂肪酸。该基因在油料作物中的种子特异性表达在转基因种子中产生了大量的DDA和DTA。然而，这些脂肪酸被排除在三酰甘油（TAGs）的-2位置。为了提高转基因油料作物中VLCPUFAs的产量水平和营养价值，从[具体来源未给出]中鉴定出一种用于将这两种脂肪酸掺入三酰甘油-2位置的细胞质溶血磷脂酸酰基转移酶（EhLPAAT2）。RT-PCR分析表明，它在发育中的种子中优先表达，而[具体基因未给出]仅在[具体部位未给出]中表达。在[具体植物未给出]中与[具体基因未给出]一起进行种子特异性表达，导致DDA和DTA有效地掺入TAGs的-2位置，从而增加了转基因种子中DDA和DTA的总量。据我们所知，这是第一种能够将VLCPUFAs掺入TAGs的植物LPAAT。利用EhLPAAT2和EhELO1提高油料作物中DDA和DTA的产量为营养保健品高价值农产品提供了真正的商业机会。
