Cao Yunpeng, Li Qiang, Zhang Lin
School of Health and Nursing, Wuchang University of Technology, Wuhan, China.
Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China.
Front Plant Sci. 2023 Apr 3;14:1170723. doi: 10.3389/fpls.2023.1170723. eCollection 2023.
Woody oil plants are the most productive oil-bearing species that produce seeds with high levels of valuable triacylglycerols (TAGs). TAGs and their derivatives are the raw materials for many macromolecular bio-based products, such as nylon precursors, and biomass-based diesel. Here, we identified 280 genes encoding seven distinct classes of enzymes (i.e., G3PAT, LPAAT, PAP, DGAT, PDCT, PDAT, and CPT) involved in TAGs-biosynthesis. Several multigene families are expanded by large-scale duplication events, such as , and . RNA-seq was used to survey the expression profiles of these TAG pathway-related genes in different tissues or development, indicating functional redundancy for some duplicated genes originated from the large-scale duplication events, and neo-functionalization or sub-functionalization for some of them. Sixty-two genes showed strong, preferential expression during the period of rapid seed lipid synthesis, suggesting that their might represented the core TAG-toolbox. We also revealed for the first time that there is no PDCT pathway in and . The identification of key genes involved in lipid biosynthesis will be the foundation to plan strategies to develop woody oil plant varieties with enhanced processing properties and high oil content.
木本油料植物是最具生产力的产油物种，其种子富含高含量的有价值的三酰甘油（TAGs）。TAGs及其衍生物是许多大分子生物基产品的原料，如尼龙前体和生物质基柴油。在此，我们鉴定了280个编码参与TAGs生物合成的七类不同酶（即甘油-3-磷酸酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、二酰甘油酰基转移酶、磷脂：二酰甘油胆碱磷酸转移酶、磷脂：二酰甘油酰基转移酶和胆碱磷酸转移酶）的基因。几个多基因家族通过大规模复制事件得以扩展，如[具体复制事件未给出]和[具体复制事件未给出]。RNA测序用于调查这些TAG途径相关基因在不同组织或发育阶段的表达谱，表明一些源自大规模复制事件的重复基因存在功能冗余，其中一些基因发生了新功能化或亚功能化。62个基因在种子脂质快速合成期间表现出强烈的、优先的表达，这表明它们可能代表了核心TAG工具箱。我们还首次揭示了[具体物种未给出]和[具体物种未给出]中不存在磷脂：二酰甘油胆碱磷酸转移酶途径。鉴定参与脂质生物合成的关键基因将为制定策略以培育具有优良加工特性和高油含量的木本油料植物品种奠定基础。
