Sangkana Suthinee, Eawsakul Komgrit, Ongtanasup Tassanee, Boonhok Rachasak, Mitsuwan Watcharapong, Chimplee Siriphorn, Paul Alok K, Saravanabhavan Shanmuga Sundar, Mahboob Tooba, Nawaz Muhammad, Pereira Maria L, Wilairatana Polrat, Wiart Christophe, Nissapatorn Veeranoot
School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team), World Union for Herbal Drug Discovery (WUHeDD), Research Excellence Center for Innovation and Health Products (RECIHP), Walailak University Nakhon Si Thammarat 80160 Thailand
School of Medicine, Walailak University Nakhon Si Thammarat 80160 Thailand.
Nanoscale Adv. 2024 Jan 9;6(5):1467-1479. doi: 10.1039/d3na01016c. eCollection 2024 Feb 27.
extract (GME) has severe pharmacokinetic deficiencies and is made up of a variety of bioactive components. GME has proven its anti- effectiveness. In this investigation, a GME-loaded niosome was developed to increase its potential therapeutic efficacy. A GME-loaded niosome was prepared by encapsulation in a mixture of span60, cholesterol, and chloroform by the thin film hydration method. The vesicle size, zeta potential, percentage of entrapment efficiency, and stability of GME-loaded niosomes were investigated. The values for GME-loaded niosome size and zeta potential were 404.23 ± 4.59 and -32.03 ± 0.95, respectively. The delivery system enhanced the anti- activity, which possessed MIC values of 0.25-4 mg mL. In addition, the niosomal formulation decreased the toxicity of GME by 16 times. GME-loaded niosome must be stored at 4 °C, as the quantity of remaining GME encapsulated is greater at this temperature than at room temperature. SEM revealed the damage to the cell membrane caused by trophozoites and cysts, which led to dead cells. In light of the above, it was found that GME-loaded niosomes had better anti- activity. The study suggested that GME-loaded niosomes could be used as an alternative to 's therapeutic effects.
提取物（GME）存在严重的药代动力学缺陷，且由多种生物活性成分组成。GME已证明其具有抗[具体作用]效果。在本研究中，开发了一种载GME的脂质体以提高其潜在治疗效果。通过薄膜水化法将GME包裹于司盘60、胆固醇和氯仿的混合物中制备载GME的脂质体。研究了载GME脂质体的囊泡大小、zeta电位、包封率百分比和稳定性。载GME脂质体的大小和zeta电位值分别为404.23±4.59和 -32.03±0.95。该递送系统增强了抗[具体活性]，其MIC值为0.25 - 4 mg/mL。此外，脂质体制剂使GME的毒性降低了16倍。载GME脂质体必须保存在4°C，因为在此温度下包封的剩余GME量比室温下更多。扫描电子显微镜显示滋养体和包囊对细胞膜造成的损伤，导致细胞死亡。综上所述，发现载GME脂质体具有更好的抗[具体活性]。该研究表明载GME脂质体可作为[具体治疗效果]的替代物。 （注：原文中部分关键信息缺失，已按格式要求完整翻译）
