School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team), World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center for Innovation and Health Products (RECIHP), Walailak University, Nakhon Si Thammarat, Thailand; Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, Thailand.
School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team), World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center for Innovation and Health Products (RECIHP), Walailak University, Nakhon Si Thammarat, Thailand.
Int J Parasitol Drugs Drug Resist. 2020 Dec;14:218-229. doi: 10.1016/j.ijpddr.2020.11.001. Epub 2020 Nov 17.
Curcuma longa and Curcumin have been documented to have a wide spectrum of pharmacological effects, including anti-Acanthamoeba activity. Hence, this study sought to explore the anti-adhesion activity of C. longa extract and Curcumin against Acanthamoeba triangularis trophozoites and cysts in plastic and contact lenses. Our results showed that C. longa extract and Curcumin significantly inhibited the adhesion of A. triangularis trophozoites and cysts to the plastic surface, as investigated by the crystal violet assay (P < 0.05). Also, an 80-90% decrease in adhesion of trophozoites and cysts to the plastic surface was detected following the treatment with C. longa extract and Curcumin at 1/2 × MIC, compared to the control. In the contact lens model, approximately 1 log cells/mL of the trophozoites and cysts was reduced when the cells were treated with Curcumin, when compared to the control. Pre-treatment of the plastic surface with Curcumin at 1/2-MIC reduced 60% and 90% of the adhesion of trophozoites and cysts, respectively. The reduction in 1 Log cells/mL of the adhesion of A. triangularis trophozoites was observed when lenses were pre-treated with both the extract and Curcumin. Base on the results obtained from this study, A. triangularis trophozoites treated with C. longa extract and Curcumin have lost strong acanthopodia, thorn-like projection pseudopodia observed by scanning electron microscope. This study also revealed the therapeutic potentials of C. longa extract and Curcumin, as such, have promising anti-adhesive potential that can be used in the management/prevention of A. triangularis adhesion to contact lenses.
姜黄和姜黄素已被证明具有广泛的药理作用，包括抗棘阿米巴活性。因此，本研究旨在探索姜黄提取物和姜黄素对棘阿米巴三角原虫滋养体和包囊在塑料和隐形眼镜上的抗黏附活性。我们的结果表明，姜黄提取物和姜黄素通过结晶紫测定法显著抑制了棘阿米巴三角原虫滋养体和包囊对塑料表面的黏附（P<0.05）。此外，在 1/2 MIC 时，姜黄提取物和姜黄素处理后，滋养体和包囊对塑料表面的黏附减少了 80-90%，与对照组相比。在隐形眼镜模型中，与对照组相比，当用姜黄素处理时，滋养体和包囊的对数减少了约 1 个对数细胞/mL。在 1/2-MIC 时用姜黄素预处理塑料表面，分别减少了 60%和 90%的滋养体和包囊黏附。当镜片用提取物和姜黄素预处理时，观察到棘阿米巴三角原虫滋养体的黏附减少了 1 个对数细胞/mL。基于本研究的结果，用姜黄提取物和姜黄素处理的棘阿米巴三角原虫失去了强烈的棘毛，扫描电子显微镜观察到刺状突起伪足。本研究还揭示了姜黄提取物和姜黄素的治疗潜力，因此，具有有前途的抗黏附潜力，可用于管理/预防棘阿米巴附着在隐形眼镜上。
