Department of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev.
Department of Anesthesiology and Perioperative Medicine, Mayo Clinic.
J Vis Exp. 2021 May 7(171). doi: 10.3791/62291.
Impairments to sensory, short-term, and long-term memory are common side effects after traumatic brain injury (TBI). Due to the ethical limitations of human studies, animal models provide suitable alternatives to test treatment methods, and to study the mechanisms and related complications of the condition. Experimental rodent models have historically been the most widely used due to their accessibility, low cost, reproducibility, and validated approaches. A metric test, which tests the ability to recall the placement of two objects at various distances and angles from one another, is a technique to study impairment in spatial working memory (SWM) after TBI. The significant advantages of metric tasks include the possibility of dynamic observation, low cost, reproducibility, relative ease of implementation, and low stress environment. Here, we present a metric test protocol to measure impairment of SWM in adult rats after TBI. This test provides a feasible way to evaluate physiology and pathophysiology of brain function more effectively.
感觉、短期和长期记忆障碍是创伤性脑损伤 (TBI) 后的常见副作用。由于人类研究的伦理限制，动物模型为测试治疗方法提供了合适的替代品，并研究了该疾病的机制和相关并发症。由于其可及性、低成本、可重复性和经过验证的方法，实验啮齿动物模型历来是最广泛使用的模型。一种度量测试，用于测试在不同距离和角度下回忆两个物体放置位置的能力，是研究 TBI 后空间工作记忆 (SWM) 损伤的一种技术。度量任务的显著优势包括动态观察的可能性、低成本、可重复性、相对容易实施以及低压力环境。在这里，我们提出了一种度量测试方案，用于测量 TBI 后成年大鼠的 SWM 损伤。该测试提供了一种更有效地评估大脑功能生理学和病理生理学的可行方法。
