Centro Nacional de Biotecnología-CSIC, Madrid, Spain.
DISAFA-PlantStressLab, University of Turin, Grugliasco (TO), Italy.
Methods Mol Biol. 2021;2309:191-200. doi: 10.1007/978-1-0716-1429-7_15.
The binding of strigolactones to their receptor, the α/β hydrolase DWARF14 (D14), leads to the modulation of transcriptional activity by destabilization of specific transcriptional corepressors via proteasomal degradation. Subsequently, strigolactones also promote D14 degradation by the same pathway. Here we describe an innovative quantitative bioassay based on Arabidopsis transgenic lines expressing AtD14 fused to the firefly luciferase, developed to identify new strigolactone analogs capable to activate the strigolactone signaling.
独脚金内酯与受体（α/β 水解酶 DWARF14（D14））结合，导致通过蛋白酶体降解使特定转录共阻遏物失稳，从而调节转录活性。随后，独脚金内酯也通过相同途径促进 D14 的降解。在这里，我们描述了一种基于拟南芥转基因系的创新定量生物测定方法，该方法表达了与萤火虫荧光素融合的 AtD14，用于鉴定能够激活独脚金内酯信号的新的独脚金内酯类似物。
