Department of Medical parasitology, Faculty of medicine, Zagazig University, Zagazig44159, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig44159, Egypt.
J Helminthol. 2021 May 25;95:e26. doi: 10.1017/S0022149X21000171.
Trichinellosis is a zoonotic disease that endangers human health and can lead to death. Restricted absorption and poor results of conventional therapies demand new effective natural remedies to treat both enteral and parenteral trichinellosis. This study assessed the antiparasitic and anti-inflammatory effects of Citrus limon and Capsicum frutescens on murine trichinellosis and compared them with those of albendazole and prednisolone, which are conventionally used to treat trichinellosis. Overall, 50 Swiss albino male mice were divided into five groups, with ten mice in each group: negative control, positive control, albendazole combined with prednisolone, C. limon, and C. frutescens. Mice were sacrificed 7 and 35 days after infection, for intestinal and muscular phase analyses. Drug efficacies were parasitologically, biochemically, histopathologically and ultrastructurally assessed. Our results demonstrated the efficacy of C. frutescens and C. limon extracts as antiparasitic agents, showing a substantial decrease in adult and larval counts. Moreover, both extracts had the ability to decrease serum tumour necrosis factor-α levels during the intestinal and muscular phases. In addition to the improved histopathological changes in the small intestine and muscles, the destructive effects on adults and larvae were ultrastructurally evident on transmission electron microscopy. In conclusion, C. frutescens and C. limon extracts are promising remedies for the treatment of experimental trichinellosis, particularly, the C. frutescens extract.
旋毛虫病是一种危害人类健康的人畜共患病，可导致死亡。常规治疗方法的吸收受限和效果不佳，需要新的有效天然药物来治疗肠内和肠外旋毛虫病。本研究评估了柠檬和辣椒对小鼠旋毛虫病的驱虫和抗炎作用，并将其与常用于治疗旋毛虫病的阿苯达唑和泼尼松龙进行了比较。总体而言，将 50 只瑞士白化雄性小鼠分为五组，每组 10 只：阴性对照组、阳性对照组、阿苯达唑联合泼尼松龙组、柠檬组和辣椒组。感染后第 7 天和第 35 天处死小鼠，进行肠道和肌肉期分析。从寄生虫学、生化、组织病理学和超微结构评估药物疗效。我们的结果表明，柠檬和辣椒提取物作为驱虫剂具有疗效，可显著减少成虫和幼虫的数量。此外，两种提取物均能降低血清肿瘤坏死因子-α水平，无论是在肠道期还是肌肉期。除了小肠和肌肉的组织病理学变化得到改善外，电镜下也可明显观察到对成虫和幼虫的破坏作用。总之，柠檬和辣椒提取物是治疗实验性旋毛虫病的有前途的药物，特别是辣椒提取物。
