Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig 44519, Egypt.
Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.
J Helminthol. 2022 Jul 20;96:e50. doi: 10.1017/S0022149X22000359.
Trichinosis is a serious zoonotic disease that causes human morbidity and mortality. New effective natural remedies with minimal side effects that are well tolerated are needed to treat both enteral and parenteral trichinosis. This study evaluated the efficacy of selenium (Se), Se nanoparticles (SeNPs) and Egyptian propolis compared with albendazole as antiparasitic, anti-inflammatory and anti-angiogenic agents for treating murine trichinosis. We used parasitological, histopathological and immunohistochemical assays, as well as scanning electron microscopy, to examine adult worms. Overall, 80 Swiss albino male mice were divided into eight groups, with ten mice in each group, as follows: negative control, positive control, albendazole, propolis, Se, combination of propolis and Se, SeNPs and combination of SeNPs and propolis. Mice were slaughtered seven and 35 days after infection to examine the intestinal and muscular phases, respectively. This study demonstrated the efficacy of the combination of SeNPs and propolis. As revealed by electron microscopy, this combination caused damage to the adult worm cuticle. Additionally, compared with albendazole, it resulted in a significant reduction in adult worm and total larval counts; moreover, it caused a decrease in the number of larvae deposited in muscles, with a highly significant decrease in the inflammatory cell infiltrate around the larvae and a considerable decrease in the expression of the angiogenic marker vascular endothelial growth factor in muscles. In conclusion, the combination of SeNPs and propolis had antiparasitic, anti-inflammatory and anti-angiogenic effects on trichinosis. Consequently, this combination could be used as a natural alternative therapy to albendazole for treating trichinosis.
旋毛虫病是一种严重的人畜共患疾病，可导致人类发病和死亡。需要寻找新的、有效的、副作用小、耐受性好的天然药物来治疗肠内和肠外旋毛虫病。本研究评估了硒（Se）、硒纳米颗粒（SeNPs）和埃及蜂胶与阿苯达唑相比作为抗寄生虫、抗炎和抗血管生成药物治疗小鼠旋毛虫病的疗效。我们使用寄生虫学、组织病理学和免疫组织化学检测以及扫描电子显微镜检查了成虫。总体而言，将 80 只瑞士白化雄性小鼠分为 8 组，每组 10 只，如下所示：阴性对照、阳性对照、阿苯达唑、蜂胶、Se、蜂胶和 Se 的组合、SeNPs 和 SeNPs 与蜂胶的组合。感染后 7 天和 35 天分别宰杀小鼠，检查肠道期和肌肉期。本研究证明了 SeNPs 和蜂胶联合应用的疗效。电镜观察显示，这种组合导致成虫表皮受损。此外，与阿苯达唑相比，它显著减少了成虫和总幼虫数量；此外，它减少了肌肉中幼虫的沉积数量，幼虫周围的炎症细胞浸润显著减少，肌肉中血管内皮生长因子等血管生成标志物的表达也显著降低。总之，SeNPs 和蜂胶联合应用对旋毛虫病具有抗寄生虫、抗炎和抗血管生成作用。因此，这种组合可能作为治疗旋毛虫病的阿苯达唑的天然替代疗法。
