College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
Anal Chem. 2021 Jun 8;93(22):7949-7957. doi: 10.1021/acs.analchem.1c00731. Epub 2021 May 25.
Real-time and in situ visualization of cerebrovascular dysfunction is significant for studying brain injury, which however, is restricted by the complex brain structure and limited imaging strategies. Luminescence imaging in NIR-IIb region (1500-1700 nm) is a promising tool owing to its merits including deep penetration, high resolution, and fast data acquisition. Unfortunately, a luminescent material in this region with sufficient brightness and biocompatibility is scarce. Herein, AgSe quantum dot-sensitized lanthanide-doped nanocrystals (QDs-LnNCs) with emission beyond 1500 nm were fabricated to image the cerebrovascular structure and hemodynamics in ischemic stroke and traumatic brain injury. The sensitization by QDs provided an over 100-fold enhanced brightness of LnNCs and a remarkable penetration depth of 11 mm. Dynamic information of blood perfusion and flow rates were acquired and the damage of the blood-brain barrier in the two injury models was investigated. Our results proved QDs-LnNCs as a kind of competent nanomaterial for noninvasive brain imaging.
实时和原位可视化脑血管功能对于研究脑损伤具有重要意义，但受到复杂的大脑结构和有限的成像策略的限制。近红外二区（1500-1700nm）的荧光成像是一种很有前途的工具，因为它具有穿透深度深、分辨率高、数据采集速度快等优点。不幸的是，在这个区域内，具有足够亮度和生物相容性的发光材料却很稀缺。在此，我们制备了发射波长超过 1500nm 的 AgSe 量子点敏化的镧系掺杂纳米晶（QDs-LnNCs），用于在缺血性中风和创伤性脑损伤中对脑血管结构和血液动力学进行成像。QDs 的敏化作用使 LnNCs 的亮度提高了 100 多倍，穿透深度达到 11mm。我们获取了灌注和血流速率的动态信息，并研究了两种损伤模型中的血脑屏障损伤。我们的研究结果证明了 QDs-LnNCs 是一种用于非侵入性脑成像的有潜力的纳米材料。
