Department of Medical Biophysics, Kobe University Graduate School of Health Sciences, Kobe, Japan.
Department of Diagnostic Pathology, Kobe University Graduate School of Medicine, Kobe, Japan.
Cytopathology. 2021 Sep;32(5):654-659. doi: 10.1111/cyt.12998. Epub 2021 Jun 10.
In The Paris System for Reporting Urinary Cytology (TPS), the important cytomorphological features for diagnosing high-grade urothelial carcinoma (HGUC) are a nuclear-to-cytoplasmic (N:C) ratio exceeding 0.7, hyperchromasia, coarse chromatin, and irregular nuclear borders. However, quantitative cytomorphological assessments of HGUC cells using SurePath slides are rare. Therefore, we evaluated HGUC cells on SurePath slides quantitatively using a digital image analysis system and compared these data with ThinPrep data.
The same urine samples were divided into two aliquots and used to prepare SurePath and ThinPrep slides. We used ImageJ to measure the N:C ratio, hyperchromasia, and irregular nuclear borders for HGUC cells on SurePath and ThinPrep slides.
The total number of analysed HGUC cells on SurePath slides was 981, versus 889 on ThinPrep slides. Hyperchromasia and irregular nuclear borders were significantly more severe on SurePath than on ThinPrep slides. Conversely, the N:C ratio did not differ between the methods. Additionally, HGUC cells with N:C ratios exceeding 0.7 were present on almost all slides for both methods.
Our data indicated the reasonableness of using the N:C ratio as the major criterion for TPS on both SurePath and ThinPrep slides, and an N:C ratio cut-off of 0.7 as suitable for identifying HGUC cells. However, the severity of hyperchromasia and irregular nuclear borders differed between the processing methods.
在巴黎泌尿系统细胞学报告系统（TPS）中，诊断高级别尿路上皮癌（HGUC）的重要细胞学形态特征是核质比（N：C）超过 0.7、核浆比增高、粗染色质和不规则核边界。然而，使用 SurePath 载玻片对 HGUC 细胞进行定量细胞学评估较为少见。因此，我们使用数字图像分析系统对 SurePath 载玻片上的 HGUC 细胞进行定量评估，并将这些数据与 ThinPrep 数据进行比较。
将相同的尿液样本分为两份，分别用于制备 SurePath 和 ThinPrep 载玻片。我们使用 ImageJ 测量 SurePath 和 ThinPrep 载玻片上 HGUC 细胞的 N：C 比值、核浆比增高和不规则核边界。
SurePath 载玻片上分析的 HGUC 细胞总数为 981 个，而 ThinPrep 载玻片上为 889 个。SurePath 载玻片上的核浆比增高和不规则核边界明显比 ThinPrep 载玻片上严重。相反，两种方法的 N：C 比值无差异。此外，两种方法的载玻片上几乎都存在 N：C 比值超过 0.7 的 HGUC 细胞。
我们的数据表明，在 SurePath 和 ThinPrep 载玻片上使用 N：C 比值作为 TPS 的主要标准是合理的，N：C 比值为 0.7 作为识别 HGUC 细胞的合适标准。然而，两种处理方法的核浆比增高和不规则核边界的严重程度存在差异。
