Fay Petra, Limon Georgina, Ulziibat Gerelmaa, Khanui Buyantogtokh, Myagmarsuren Odonchimeg, Tore Gessica, Khishgee Bodisaikhan, Flannery John, Sandag Batkhuyag, Damdinjav Batchuluun, Beard Philippa M
The Pirbright Institute, Pirbright, UK.
State Central Veterinary Laboratory, Zaisan, Khan-Uul District, Ulaanbaatar, Mongolia.
Transbound Emerg Dis. 2022 Jul;69(4):1837-1846. doi: 10.1111/tbed.14163. Epub 2021 Jun 25.
Sheeppox is a transboundary disease of small ruminants caused by infection with the capripoxvirus sheeppox virus. Sheeppox is found in Africa, the Middle East and Asia and is characterized by fever, multifocal cutaneous raised lesions and death. Vaccination with live attenuated capripoxvirus (CPPV) strains is an effective and widely used strategy to contol sheeppox outbreaks; however, there are few reports of post-vaccination field surveillance studies. This study used a commercially available enzyme-linked immunosorbent assay (ELISA) to examine quantitative and temporal features of the humoral response of sheep vaccinated with a live-attenuated CPPV strain in Mongolia. Four hundred samples were tested using the ELISA commercial kit, and a subset of 45 samples were also tested with a virus neutralization test (VNT). There was substantial agreement between the VNT and ELISA tests. Antibodies to CPPV were detected between 40 and 262 days post-vaccination. There was no significant difference between serological status (positive/negative) and sex or age; however, an inverse correlation was found between the length of time since vaccination and serological status. Animals between 90 and 180 days post-vaccination were more likely to be positive than animals greater than 180 days post-vaccination. Our results show that a commercial CPPV ELISA kit is a robust and reliable assay for post-CPPV vaccination surveillance in resource-restricted settings and provide temporal parameters to be considered when planning sheeppox post-vaccination monitoring programmes.
绵羊痘是由山羊痘病毒属绵羊痘病毒感染引起的小反刍兽类跨界疾病。绵羊痘在非洲、中东和亚洲均有发现，其特征为发热、多灶性皮肤隆起性病变及死亡。使用减毒活山羊痘病毒（CPPV）毒株进行疫苗接种是控制绵羊痘疫情爆发的一种有效且广泛应用的策略；然而，关于疫苗接种后现场监测研究的报道较少。本研究使用一种市售酶联免疫吸附测定（ELISA）方法，来检测在蒙古国接种减毒活CPPV毒株的绵羊体液免疫反应的定量和时间特征。使用ELISA商用试剂盒检测了400份样本，并使用病毒中和试验（VNT）对45份样本的子集进行了检测。VNT和ELISA检测结果之间存在高度一致性。在接种疫苗后40至262天之间检测到了针对CPPV的抗体。血清学状态（阳性/阴性）与性别或年龄之间无显著差异；然而，发现自接种疫苗后的时间长度与血清学状态呈负相关。接种疫苗后90至180天的动物比接种疫苗后超过180天的动物更有可能呈阳性。我们的结果表明，市售CPPV ELISA试剂盒是在资源有限环境中进行CPPV疫苗接种后监测的一种稳健且可靠的检测方法，并为规划绵羊痘疫苗接种后监测计划时应考虑的时间参数提供了依据。
