Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, China.
J Ethnopharmacol. 2021 Sep 15;277:114215. doi: 10.1016/j.jep.2021.114215. Epub 2021 May 24.
Hypoxia will cause an increase in the rate of fatigue and aging. Chinese cordyceps, a parasitic Thitarodes insect-Ophiocordyceps sinensis fungus complex in the Qinghai-Tibet Plateau, has long been used to ameliorate human conditions associated with aging and senescence, it is principally applied to treat fatigue, night sweating and other symptoms related to aging, and it may play the anti-aging and anti-fatigue effect by improving the body's hypoxia tolerance.
The present study investigated the anti-hypoxia activity of Chinese cordyceps and explore the main corresponding signal pathways and bioactive compounds.
In this study, network pharmacology analysis, molecular docking, cell and whole pharmacodynamic experiments were hired to study the major signal pathways and the bioactive compounds of Chinese cordyceps for anti-hypoxia activity.
17 pathways which Chinese cordyceps acted on seemed to be related to the anti-hypoxia effect, and "VEGF signal pathway" was one of the most important pathway. Chinese cordyceps improved the survival rate and regulated the targets related VEGF signal pathway of H9C2 cells under hypoxia, and also had significant anti-hypoxia effects to mice. Chorioallantoic membrane model experiment showed that Chinese cordyceps and the main constituents of (9Z,12Z)-octadeca-9,12-dienoic acid and cerevisterol had significant angiogenic activity in hypoxia condition.
Based on the results of network pharmacology and molecular docking analysis, cell and whole pharmacodynamic experiments, promoting angiogenesis by regulating VEGF signal pathway might be one of the mechanisms of anti-hypoxia effect of Chinese cordyceps, (9Z, 12Z)-octadeca-9,12-dienoic acid and cerevisterol were considered as the major anti-hypoxia bioactive compounds in Chinese cordyceps.
缺氧会导致疲劳和衰老的速度加快。中国虫草，一种寄生在青藏高原的 Thitarodes 昆虫-中华被毛孢真菌复合体，长期以来一直被用于改善与衰老和衰老相关的人体状况，主要用于治疗疲劳、夜间出汗和其他与衰老相关的症状，它可能通过提高身体的耐缺氧能力发挥抗衰老和抗疲劳作用。
本研究调查了中国虫草的抗缺氧活性，并探讨了主要的相应信号通路和生物活性化合物。
在这项研究中，网络药理学分析、分子对接、细胞和整体药效学实验被用来研究中国虫草抗缺氧活性的主要信号通路和生物活性化合物。
中国虫草作用的 17 条通路似乎与抗缺氧作用有关，其中“VEGF 信号通路”是最重要的通路之一。中国虫草在缺氧条件下提高了 H9C2 细胞的存活率，并调节了与 VEGF 信号通路相关的靶点，对小鼠也有显著的抗缺氧作用。鸡胚尿囊膜模型实验表明，中国虫草及其主要成分（9Z，12Z）-十八碳-9,12-二烯酸和麦角固醇在缺氧条件下具有显著的血管生成活性。
基于网络药理学和分子对接分析、细胞和整体药效学实验的结果，通过调节 VEGF 信号通路促进血管生成可能是中国虫草抗缺氧作用的机制之一，（9Z，12Z）-十八碳-9,12-二烯酸和麦角固醇被认为是中国虫草中的主要抗缺氧生物活性化合物。
