School of Dentistry and Oral Health, Griffith University, Gold Coast, QLD, SA.
School of Dentistry, The University of Queensland, Queensland, SA.
Photodiagnosis Photodyn Ther. 2021 Sep;35:102344. doi: 10.1016/j.pdpdt.2021.102344. Epub 2021 May 24.
This study aimed to assess changes in the fluorescence characteristics of Enterococcus faecalis in human dentine over a period of 24 h following treatment with endodontic irrigants.
Sterilised, non-functional extracted third molars were embedded in acrylic resin and uniformly sectioned into 2 mm thick dentine sections. After the removal of smear layer, the dentine sections were inoculated with E. faecalis and cultured for 7 days. The infected dentine sections were subsequently treated with different concentrations of sodium hypochlorite (NaOCl) and hydrogen peroxide (HO). Bacterial fluorescence readings were assessed at different time points using a calibrated laser device. All data were assessed for normality (Kolmogorov Smirnoff test) and analysed using ANOVA with Bonferroni post-hoc tests.
Fluorescence readings were quenched when E. faecalis infected human dentine sections were treated with oxidizing irrigants in vitro. Throughout a 24-hour period, fluorescence recovered in part but did not return to baseline level.
The fluorescence quenching effect of these oxidizing agents needs to be considered when using laser fluorescence in assessing the quality of root canal debridement or disinfection.
本研究旨在评估在根管冲洗剂处理后 24 小时内，粪肠球菌在人牙本质中的荧光特性变化。
将消毒的、非功能性的第三磨牙嵌入丙烯酸树脂中，并均匀地切成 2 毫米厚的牙本质片。去除玷污层后，将牙本质片接种粪肠球菌并培养 7 天。随后用不同浓度的次氯酸钠（NaOCl）和过氧化氢（HO）处理感染的牙本质片。使用校准的激光设备在不同时间点评估细菌荧光读数。使用方差分析和 Bonferroni 事后检验对所有数据进行正态性检验（Kolmogorov Smirnov 检验）。
体外用氧化冲洗剂处理感染人牙本质片时，荧光被猝灭。在 24 小时内，荧光部分恢复，但未恢复到基线水平。
在使用激光荧光评估根管清创或消毒质量时，需要考虑这些氧化剂的荧光猝灭效应。
