School of Dentistry, University of Queensland, Herston, QLD, Australia.
School of Medcine and Dentistry, Griffith University, Southport, Queensland, Australia.
Photodiagnosis Photodyn Ther. 2022 Mar;37:102651. doi: 10.1016/j.pdpdt.2021.102651. Epub 2021 Nov 24.
This study examined the effect of various root canal irrigants and medicaments on dentin fluorescence elicited by 655 nm visible red laser light. To replicate clinical use, irrigants were applied onto dentin samples for 2 min, while medicaments were applied for 2 weeks. Fluorescence values tracked from baseline across the following to 24 h, starting 5 min after exposure. Sodium hypochlorite, hydrogen peroxide, and articaine local anaesthetic (4% articaine with 1:1000,000 adrenaline) all significantly quenched fluorescence (p < 0.0001), which then returned to baseline levels after 20 min. Conversely, elevated fluorescence readings were recorded after 3% mepivacaine (p < 0.05), 0.2% chlorhexidine (p < 0.01) and chloroform (p <0.05). A 2 week application of Ledermix™ paste containing 3% demeclocycline caused an irreversible increase in fluorescence (p < 0.0001). Other tested endodontic materials (15% EDTA, eucalyptus oil, calcium hydroxide, Odontopaste™ clindamycin paste, and distilled water) had no impact on dentine fluorescence. The influences of endodontic materials on dentin fluorescence need to considered when using fluorescence endpoints to guide the progress of root canal treatment.
本研究旨在探讨不同根管冲洗剂和药物对 655nm 可见红光激发牙本质荧光的影响。为了模拟临床应用，将冲洗剂应用于牙本质样本上 2 分钟，而药物则应用 2 周。荧光值从基线开始跟踪，在暴露后 5 分钟开始，持续 24 小时。次氯酸钠、过氧化氢和阿替卡因局部麻醉剂（4%阿替卡因加 1:1000000 肾上腺素）均显著猝灭荧光（p<0.0001），20 分钟后荧光值恢复基线水平。相反，在 3%甲哌卡因（p<0.05）、0.2%洗必泰（p<0.01）和氯仿（p<0.05）后记录到荧光读数升高。含 3%地美环素的 Ledermix™糊剂应用 2 周后，荧光强度不可逆增加（p<0.0001）。其他测试的根管材料（15%EDTA、桉树油、氢氧化钙、Odontopaste™克林霉素糊剂和蒸馏水）对牙本质荧光无影响。在使用荧光终点指导根管治疗进展时，需要考虑根管材料对牙本质荧光的影响。
