Pokhrel Anaya, Kang Sun-Young, Schmidt-Dannert Claudia
Department of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, 140 Gortner Laboratory, 1479 Gortner Avenue, Saint Paul, MN 55108, USA.
Department of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, 140 Gortner Laboratory, 1479 Gortner Avenue, Saint Paul, MN 55108, USA.
Curr Opin Microbiol. 2021 Aug;62:28-37. doi: 10.1016/j.mib.2021.04.008. Epub 2021 May 24.
Two decades of structural and functional studies have revealed functions, structures and diversity of bacterial microcompartments. The protein-based organelles encapsulate diverse metabolic pathways in semipermeable, icosahedral or pseudo-icosahedral shells. One of the first discovered and characterized microcompartments are those involved in ethanolamine degradation. This review will summarize their function and assembly along with shared and unique characteristics with other microcompartment types. The modularity and self-assembling properties of their shell proteins make them valuable targets for bioengineering. Advances and prospects for shell protein engineering in vivo and in vitro for synthetic biology and biotechnology applications will be discussed.
二十年的结构与功能研究揭示了细菌微区室的功能、结构及多样性。这些基于蛋白质的细胞器将多样的代谢途径包裹于半透性的二十面体或准二十面体外壳中。最早发现并得以表征的微区室之一是参与乙醇胺降解的微区室。本综述将总结其功能与组装过程，以及与其他微区室类型共有的和独特的特征。其外壳蛋白的模块化和自组装特性使其成为生物工程的宝贵靶点。还将讨论在体内和体外进行外壳蛋白工程以用于合成生物学和生物技术应用的进展与前景。
