Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, 100083, China; College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China.
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China.
Plant Sci. 2021 Jul;308:110908. doi: 10.1016/j.plantsci.2021.110908. Epub 2021 Apr 8.
Chloroplast biogenesis and development regulation have long been a focus of research; however, the underlying mechanisms of these processes have not yet been fully elucidated. Pentatricopeptide repeat (PPR) proteins have been shown to play key roles in chloroplast development. Here, we identified a novel P-type PPR protein, Early Chloroplast Development 2 (ECD2), and the ecd2 mutant resulted in embryo lethality. The RNAi lines of ECD2 showed varying degrees of albino cotyledons and abnormal chloroplast development, but true leaves were similar to the wild-type. Further analysis revealed that ECD2 was responsible for chloroplast gene expression and group II intron splicing of several genes. Transcriptome analysis combined with quantitative real-time PCR showed that ECD2 was associated with the expression of ribosomal genes and accumulation of chloroplast ribosomes. Overall, our results indicate that ECD2 is critically important for early chloroplast development in cotyledon.
叶绿体的生物发生和发育调控一直是研究的重点；然而，这些过程的潜在机制尚未完全阐明。五肽重复（PPR）蛋白被证明在叶绿体发育中发挥关键作用。在这里，我们鉴定了一个新的 P 型 PPR 蛋白，早期叶绿体发育 2（ECD2），并且 ecd2 突变体导致胚胎致死。ECD2 的 RNAi 系表现出不同程度的白化子叶和异常的叶绿体发育，但真叶与野生型相似。进一步的分析表明，ECD2 负责几个基因的叶绿体基因表达和组 II 内含子剪接。转录组分析结合定量实时 PCR 显示，ECD2 与核糖体基因的表达和叶绿体核糖体的积累有关。总的来说，我们的结果表明 ECD2 对于子叶中早期叶绿体发育至关重要。
