Zhang Bin, Wu Yuankang, Li Shoufan, Ren Wenjing, Yang Limei, Zhuang Mu, Lv Honghao, Wang Yong, Ji Jialei, Hou Xilin, Zhang Yangyong
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Hortic Res. 2024 Jan 10;11(3):uhae006. doi: 10.1093/hr/uhae006. eCollection 2024 Mar.
Leaf color is an important agronomic trait in cabbage ( L. var. ), but the detailed mechanism underlying leaf color formation remains unclear. In this study, we characterized a () mutant 4036Y, which has significantly reduced chlorophyll content and abnormal chloroplasts during early leaf development. Genetic analysis revealed that the yellow-green leaf trait is controlled by a single recessive gene. Map-based cloning revealed that encodes a novel nuclear-targeted P-type PPR protein, which is absent in the 4036Y mutant. Functional complementation showed that from the normal-green leaf 4036G can rescue the yellow-green leaf phenotype of 4036Y. The C-to-U editing efficiency and expression levels of , , and were significantly reduced in 4036Y than that in 4036G, and significantly increased in overexpression lines than that in 4036Y. The expression levels of many plastid- and nuclear-encoded genes associated with chloroplast development in mutant were also significantly altered. These results suggest that participates in chloroplast C-to-U editing and development, which provides rare insight into the molecular mechanism underlying leaf color formation in cabbage.
叶色是甘蓝（L. var.）的一个重要农艺性状，但叶片颜色形成的详细机制仍不清楚。在本研究中，我们鉴定了一个（）突变体4036Y，其在叶片发育早期叶绿素含量显著降低且叶绿体异常。遗传分析表明，黄绿色叶片性状由单个隐性基因控制。基于图谱的克隆表明，编码一种新型的定位于细胞核的P型PPR蛋白，该蛋白在4036Y突变体中缺失。功能互补表明，来自正常绿色叶片4036G的可以挽救4036Y的黄绿色叶片表型。与4036G相比，4036Y中、、和的C到U编辑效率和表达水平显著降低，而过表达株系中的编辑效率和表达水平比4036Y显著增加。突变体中许多与叶绿体发育相关的质体编码基因和核编码基因的表达水平也发生了显著变化。这些结果表明，参与叶绿体C到U编辑和发育，这为甘蓝叶片颜色形成的分子机制提供了难得的见解。
