Wang Lan-Ying, Liang Xiao, Zhao Jing, Wang Ying, Li Shao-Ping
University of Macau, Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, Macau, China.
Bino Beijing Limited, Beijing, China.
J AOAC Int. 2019 May 1;102(3):741-747. doi: 10.5740/jaoacint.18-0309. Epub 2018 Nov 15.
Cultured is very popular. To gain dynamic insight into activity markers in fruiting body of () in (), also named silkworm. The development stages of samples at 3, 9, 12, 19, 27, and 33 days after inoculation (DAI) were collected. HPLC coupled with diode array detection and evaporative light-scattering detection method (HPLC-DAD-ELSD) was used to determine eight makers, including six nucleosides and two carbohydrates from the samples. cultured 33 DAI with fifth star silkworm larva could accumulate higher levels of cordycepin (13.43 mg/g) than the highest reported cordycepin (8.57 g/L). The contents of cordycepin, adenosine, and trehalose were gradually increased with the formation of C fruiting bodies on silkworm larva, while mannitol was decreased. The change of guanosine was similar to uracil. Results suggested that mannitol could be accumulated in a short period during mycelium growth and could metabolize and transform into energy store and trehalose during fruit body formation. The inosine in the insect was completely utilized and transformed. The synergistic formation of cordycepin and adenosine or differences in metabolized pathways are a great possibility according to the same trend. This research offered some reference to further find a certain regularity or metabolic mechanism.
培养物很受欢迎。为了深入动态了解家蚕（也称为桑蚕）子实体中的活性标志物。收集接种后3、9、12、19、27和33天的样品发育阶段。采用高效液相色谱-二极管阵列检测和蒸发光散射检测法（HPLC-DAD-ELSD）测定样品中的8种标志物，包括6种核苷和2种碳水化合物。与五星家蚕幼虫共培养33天的培养物积累的虫草素水平（13.43 mg/g）高于报道的最高虫草素水平（8.57 g/L）。随着家蚕幼虫上C子实体的形成，虫草素、腺苷和海藻糖的含量逐渐增加，而甘露醇含量降低。鸟苷的变化与尿嘧啶相似。结果表明，甘露醇可在菌丝体生长的短时间内积累，并在子实体形成过程中代谢转化为能量储存物和海藻糖。昆虫体内的肌苷被完全利用和转化。根据相同趋势，虫草素和腺苷的协同形成或代谢途径的差异很有可能。该研究为进一步寻找一定规律或代谢机制提供了一些参考。
