Osman Mohamed Ahmed, Suliman Mohamed Malik, Abdelrahman Hussain Mohamed, Fatahalrahman Ahmed Ibrahim
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, International University of Africa, Khartoum, 11111, Sudan.
Department of Pharmaceutics, Faculty of Pharmacy, Sudan International University, Khartoum, 11111, Sudan.
F1000Res. 2020 Aug 26;9:1050. doi: 10.12688/f1000research.24854.2. eCollection 2020.
species are one of the most important opportunistic fungal pathogens that cause both superficial and systemic infections, especially in immunocompromised individuals. Considering the sharp increase in the rate of infections, and resistance to commonly used antifungal agents in the last decades; this study was conducted to determine the rate of resistance among clinical isolates of species, and to characterize some of the resistant genes among resistant isolates collected in Khartoum. This is a cross-sectional laboratory-based study included 100 pre-screened species isolates from Khartoum state hospitals. Chromogenic media was used for isolation and/or identification. The standard disc diffusion method was performed to investigate the susceptibility to fluconazole, itraconazole, and amphotericin. Following genomic DNA extraction, the entire gene was amplified from some resistant isolates, sequenced, and further analyzed. Out of 100 clinical isolates collected, 51% were , followed by (31%), (8%), (5%), and (5%). Resistance rate was 23% for fluconazole, 4% for itraconazole, while there were no amphotericin resistant isolates detected. gene sequence reveals 15 different mutations. Among these, three (D116E, E266D, and V488I) were missense mutations; however, these substitutions do not contribute to fluconazole resistance. was found to be the most common species. Resistance against fluconazole was observed most frequently; however, mutations in are unlikely to be the reason behind fluconazole resistance among these isolates.
某物种是最重要的机会性真菌病原体之一，可引起浅表和全身感染，尤其是在免疫功能低下的个体中。考虑到在过去几十年中感染率急剧上升以及对常用抗真菌药物产生耐药性；本研究旨在确定该物种临床分离株的耐药率，并对在喀土穆收集的耐药分离株中的一些耐药基因进行表征。这是一项基于实验室的横断面研究，纳入了来自喀土穆州医院的100株预先筛选的该物种分离株。使用显色培养基进行分离和/或鉴定。采用标准纸片扩散法研究对氟康唑、伊曲康唑和两性霉素的敏感性。在提取基因组DNA后，从一些耐药分离株中扩增整个某基因，进行测序并进一步分析。在收集的100株临床分离株中，51%为某，其次是某（31%）、某（8%）、某（5%）和某（5%）。氟康唑的耐药率为23%，伊曲康唑为4%，而未检测到对两性霉素耐药的分离株。某基因序列显示有15种不同的突变。其中，三种（D116E、E266D和V488I）为错义突变；然而，这些替换并不导致对氟康唑耐药。某被发现是最常见的物种。最常观察到对氟康唑的耐药性；然而，某中的突变不太可能是这些分离株对氟康唑耐药的原因。 （注：原文中部分物种名称未给出具体中文，用“某”代替）
