Sevindik Mustafa, Akgul Hasan, Selamoglu Zeliha, Braidy Nady
Bahçe Vocational High School, Osmaniye Korkut Ata University, 80500, Osmaniye, Turkey.
Department of Biology, Faculty of Science, Akdeniz University, Antalya, Turkey.
Mycology. 2020 Dec 2;12(2):128-138. doi: 10.1080/21501203.2020.1816584.
Octaviania asterosperma (hypogeous Basidiomycota) We investigated the phenolic composition, and antioxidant, antimicrobial and antigenotoxic effects of methanol extracts of fruiting bodies from Octaviania asterosperma. The total phenolic content (ppm) of O. asterosperma was found to be catechin (54.73 ± 4.68), epicatechin (123.90 ± 8.52), caffeic acid (4.23 ± 0.97), p-hydroxybenzoic acid (37.72 ± 3.84), cinnamic acid (58.07 ± 5.40), gallic acid (56.64 ± 6.39), clorogenic acid (80.76 ± 4.92) and coumaric acid (2.45 ± 0.15). The total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were 3.410 ± 0.099 mmol/L, 7.548 ± 0.147 μmol/L and 0.221 ± 0.005 respectively. O. asterosperma showed some promising antimicrobial activity. The extract showed no genotoxic potential and attenuated hydrogen peroxide (H2O2)-induced oxidative DNA damage in neurons. Pre-treatment with O. asterosperma maintained mitochondrial function, reduced expression levels of cleaved-caspase-3 and apoptosis-inducing factor (AIF) when HT22 cells were exposed to pathophysiological concentrations of GLU (25 mM) and modulated protein kinase B (Akt), the mammalian target of rapamycin (mTOR), and the phosphotase and tensin homolog on chromosome ten (PTEN). O. asterosperma is an important food for the treatment or management of neurodegenerative disorders due to its phenolic content and potent antioxidant and anti-excitotoxic effects.
星籽奥克塔维亚菌（地下担子菌纲） 我们研究了星籽奥克塔维亚菌子实体甲醇提取物的酚类成分、抗氧化、抗菌和抗遗传毒性作用。发现星籽奥克塔维亚菌的总酚含量（ppm）为儿茶素（54.73 ± 4.68）、表儿茶素（123.90 ± 8.52）、咖啡酸（4.23 ± 0.97）、对羟基苯甲酸（37.72 ± 3.84）、肉桂酸（58.07 ± 5.40）、没食子酸（56.64 ± 6.39）、绿原酸（80.76 ± 4.92）和香豆酸（2.45 ± 0.15）。总抗氧化状态（TAS）、总氧化状态（TOS）和氧化应激指数（OSI）分别为3.410 ± 0.099 mmol/L、7.548 ± 0.147 μmol/L和0.221 ± 0.005。星籽奥克塔维亚菌显示出一些有前景的抗菌活性。该提取物没有遗传毒性潜力，并减轻了过氧化氢（H2O2）诱导的神经元氧化DNA损伤。当HT22细胞暴露于病理生理浓度的谷氨酸（25 mM）时，用星籽奥克塔维亚菌预处理可维持线粒体功能，降低裂解型半胱天冬酶 - 3和凋亡诱导因子（AIF）的表达水平，并调节蛋白激酶B（Akt）、雷帕霉素哺乳动物靶蛋白（mTOR）和10号染色体上的磷酸酶和张力蛋白同源物（PTEN）。由于其酚类成分以及强大的抗氧化和抗兴奋毒性作用，星籽奥克塔维亚菌是治疗或管理神经退行性疾病的重要食物。
