Dott H M, Harrison R A, Foster G C
J Reprod Fertil. 1979 Jan;55(1):113-24. doi: 10.1530/jrf.0.0550113.
Epididymal spermatozoa from bull, rabbit and ram were incubated in homologous epididymal plasma or seminal plasma in a buffered saline-based medium with or without serum albumin. The spermatozoa were either diluted directly into the medium or were washed first. No effect of washing was observed on the subsequent reaction of the cells to the different media. A considerable proportion of the populations of epididymal spermatozoa survived (i.e. continued to exhibit motility) for up to 22 h at 30 degrees C in the simple saline-based medium. Initially epididymal plasma had a slight stimulatory effect on sperm motility in ram and bull but it had no effect on sperm survival in any of the 3 species. Seminal plasma stimulated motility markedly in ram initially, but in all 3 species seminal plasma was detrimental to survival: in ram even a 15-min exposure to the fluid reduced survival. Serum albumin also stimulated motility; it delayed, but did not prevent, the detrimental effect of seminal plasma, although it had no effect itself on survival. The effects of epididymal plasma, seminal plasma and serum albumin on surface properties of epididymal spermatozoa, i.e. agglutination, sticking-to-glass and eosinophilia, were also noted. These varied between species and there was no correlation between these effects and the effects on motility and survival.
将公牛、兔子和公羊的附睾精子在含有或不含有血清白蛋白的基于缓冲盐水的培养基中的同源附睾血浆或精浆中孵育。精子要么直接稀释到培养基中，要么先进行洗涤。未观察到洗涤对细胞随后对不同培养基的反应有影响。相当比例的附睾精子群体在30摄氏度的简单盐水培养基中存活（即继续表现出活力）长达22小时。最初，附睾血浆对公羊和公牛的精子活力有轻微刺激作用，但对这3个物种中的任何一个的精子存活均无影响。精浆最初对公羊的精子活力有显著刺激作用，但在所有3个物种中，精浆均对精子存活有害：在公羊中，即使接触该液体15分钟也会降低存活率。血清白蛋白也能刺激精子活力；它延迟了，但没有阻止精浆的有害作用，尽管它本身对精子存活没有影响。还记录了附睾血浆、精浆和血清白蛋白对附睾精子表面特性的影响，即凝集、黏附于玻璃和嗜酸性。这些影响因物种而异，并且这些影响与对活力和存活的影响之间没有相关性。
