J T Mary Leema, T Persia Jothy, D Magesh Peter, T S Kumar, G Dharani
Marine Biotechnology Division, National Institute of Ocean Technology, (Ministry of Earth Sciences, Government of India), Velachery - Tambaram Main Road, Pallikaranai, Chennai, 600 100, India.
Biotechnol Rep (Amst). 2021 May 7;30:e00627. doi: 10.1016/j.btre.2021.e00627. eCollection 2021 Jun.
The prime challenge in seawater culture of microalgae for high value biomolecules production is presence of salt. Hence, twelve different salt removal treatments were evaluated for their impact on the lutein, total carotenoid, chlorophyll yields and fatty acid profile of marine microalgae (NIOT-74). The effectiveness of different treatments on salt removal was also visualized with the aid of Scanning Electron Microscope (SEM). Among the tested treatments, washing the algal biomass with 0.5 % HCl augmented the lutein (11.56 mg/g) and total carotenoid yield (60.88 mg/g) 1.82 and 1.86 fold respectively, in comparison to untreated control. Highest chlorophyll content (30.64 mg/g) was noticed in the distilled water wash treatment. Different salt removal treatments also impacted the fatty acid profile and degree of unsaturation of the fatty acids significantly. This study thus, signified the importance of salt removal treatments for the commercial production of biomolecules from marine microalgae cultured in natural seawater.
利用微藻海水养殖生产高价值生物分子面临的主要挑战是盐分的存在。因此，评估了12种不同的脱盐处理对海洋微藻（NIOT - 74）叶黄素、总类胡萝卜素、叶绿素产量和脂肪酸谱的影响。还借助扫描电子显微镜（SEM）观察了不同处理在脱盐方面的效果。在测试的处理中，与未处理的对照相比，用0.5%盐酸洗涤藻类生物质使叶黄素（11.56 mg/g）和总类胡萝卜素产量（60.88 mg/g）分别提高了1.82倍和1.86倍。蒸馏水洗涤处理的叶绿素含量最高（30.64 mg/g）。不同的脱盐处理也显著影响了脂肪酸谱和脂肪酸的不饱和度。因此，本研究表明了脱盐处理对于从天然海水中养殖的海洋微藻商业化生产生物分子的重要性。
