Marine Biotechnology Division, National Institute of Ocean Technology, (Ministry of Earth Sciences, Government of India), Velachery - Tambaram Main Road, Pallikaranai, Chennai 600 100, Tamil Nadu, India.
Marine Biotechnology Division, National Institute of Ocean Technology, (Ministry of Earth Sciences, Government of India), Velachery - Tambaram Main Road, Pallikaranai, Chennai 600 100, Tamil Nadu, India.
Food Chem. 2022 Mar 15;372:131151. doi: 10.1016/j.foodchem.2021.131151. Epub 2021 Sep 17.
Chloropycean microalgae are looked up as a prospective alternate source for the production of xanthophyll carotenoid lutein. Despite, the market significance and multitude of nutraceutical applications of lutein commercial production from microalgae still remains a challenge due to the prohibitive downstream cost. This necessitates innovative less energy intensive, high lutein yielding green processes. The present work presents a comprehensive study on the rapid green microwave assisted extraction (MAE) of lutein from marine chlorophycean microalgae Chlorella sorokiniana (NIOT-2). The process parameters of microwave assisted alkali pre-treatment like exposure time (ET), alkali concentration (AC) and solid (biomass): liquid (aqueous Potassium hydroxide-KOH) ratio (S: L ratio) were optimized using single factor and response surface method (RSM) experiments. The optimized conditions for microwave assisted alkali pre-treatment (ET:1.47 min; AC: 8.16 M KOH and S:L ratio of 36.8:1 (mg/mL) augmented the lutein yield (20.69 ± 1.2 mg/g) 3.26 fold when compared to conventional extraction (6.35 ± 0.44 mg/g). Lutein extracted using optimized MAE conditions was purified and characterized. Visualization of the MAE extracted algal biomass using Scanning electron microscope confirmed the effective cell disruption. X-ray diffraction (XRD) analysis of microwave assisted alkali treated biomass (83.85%) revealed a significantly higher crystallinity index when compared to untreated control (17.28%). MAE pre-treatment can thus be propounded as a suitable process for lutein extraction from marine microalgae due to its amalgamated rapidity, homogenous heating, less energy intensiveness and high extraction yield.
海洋绿藻微藻被视为生产类胡萝卜素叶黄素的有前景的替代资源。尽管叶黄素的商业生产具有市场重要性和多种营养保健品应用，但由于下游成本过高，仍然是一个挑战。这就需要创新的、低能耗、高叶黄素产量的绿色工艺。本工作对海洋绿藻微藻 Chlorella sorokiniana (NIOT-2)的快速绿色微波辅助提取 (MAE)叶黄素进行了全面研究。采用单因素和响应面法 (RSM)实验优化了微波辅助碱预处理过程参数，如暴露时间 (ET)、碱浓度 (AC) 和固液比 (S:L 比)。微波辅助碱预处理的优化条件（ET：1.47 分钟；AC：8.16 M KOH 和 S:L 比为 36.8:1 (mg/mL)）使叶黄素产率（20.69 ± 1.2 mg/g）提高了 3.26 倍，与传统提取（6.35 ± 0.44 mg/g）相比。使用优化的 MAE 条件提取的叶黄素进行了纯化和表征。使用扫描电子显微镜对 MAE 提取的藻生物质进行可视化，证实了有效的细胞破坏。与未经处理的对照相比，微波辅助碱处理生物质（83.85%）的 X 射线衍射 (XRD)分析显示出明显更高的结晶度指数（17.28%）。因此，MAE 预处理可以作为一种从海洋微藻中提取叶黄素的合适工艺，因为它具有快速、均匀加热、低能耗和高提取产率的特点。
