Liu Panmiao, Mu Zhongde, Ji Muhuo, Liu Xiaojiang, Gu Hanwen, Peng Yi, Yang Jianjun, Xie Zhuoying, Zheng Fuyin
Department of Anesthesiology, Pain and Perioperative Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China 450052.
Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing 210009, China.
Research (Wash D C). 2021 May 4;2021:9851609. doi: 10.34133/2021/9851609. eCollection 2021.
Photonic crystal (PC) barcodes are a new type of spectrum-encoding microcarriers used in multiplex high-throughput bioassays, such as broad analysis of biomarkers for clinical diagnosis, gene expression, and cell culture. Unfortunately, most of these existing PC barcodes suffered from undesired features, including difficult spectrum-signal acquisition, weak mechanical strength, and high ontology fluorescence, which limited their development to real applications. To address these limitations, we report a new type of structural color-encoded PC barcodes. The barcodes are fabricated by the assembly of monodisperse polydopamine- (PDA-) coated silica (PDA@SiO) nanoparticles using a droplet-based microfluidic technique and followed by pyrolysis of PDA@SiO (C@SiO) barcodes. Because of the templated carbonization of adhesive PDA, the prepared C@SiO PC beads were endowed with simultaneous easy-to-identify structural color, high mechanical strength, and ultralow ontology fluorescence. We demonstrated that the structural colored C@SiO barcodes not only maintained a high structural stability and good biocompatibility during the coculturing with fibroblasts and tumor cells capture but also achieved an enhanced fluorescent-reading signal-to-noise ratio in the fluorescence-reading detection. These features make the C@SiO PC barcodes versatile for expansive application in fluorescence-reading-based multibioassays.
光子晶体（PC）条形码是一种新型的光谱编码微载体，用于多重高通量生物检测，如用于临床诊断、基因表达和细胞培养的生物标志物的广泛分析。不幸的是，这些现有的PC条形码大多存在不理想的特性，包括光谱信号采集困难、机械强度弱和本体荧光高，这限制了它们在实际应用中的发展。为了解决这些限制，我们报道了一种新型的结构颜色编码PC条形码。这些条形码是通过使用基于液滴的微流控技术组装单分散聚多巴胺（PDA）包覆的二氧化硅（PDA@SiO）纳米颗粒，然后对PDA@SiO（C@SiO）条形码进行热解制备而成。由于粘性PDA的模板碳化作用，制备的C@SiO PC微珠具有易于识别的结构颜色、高机械强度和超低本体荧光。我们证明，结构彩色的C@SiO条形码不仅在与成纤维细胞共培养和捕获肿瘤细胞的过程中保持了高结构稳定性和良好的生物相容性，而且在荧光读取检测中实现了增强的荧光读取信噪比。这些特性使得C@SiO PC条形码在基于荧光读取的多重生物检测中具有广泛的应用前景。
