Department of Hematology and Oncology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Biosens Bioelectron. 2018 Feb 15;100:404-410. doi: 10.1016/j.bios.2017.09.032. Epub 2017 Sep 20.
Rapid and sensitive diagnosing hematological infections based on the separation and detection of pathogenic bacteria in the patient's blood is a significant challenge. To address this, we herein present a new barcodes technology that can simultaneously capture and detect multiple types of pathogenic bacteria from a complex sample. The barcodes are poly (ethylene glycol) (PEG) hydrogel inverse opal particles with characteristic reflection peak codes that remain stable during bacteria capture on their surfaces. As the spherical surface of the particles has ordered porous nanostructure, the barcodes can provide not only more surface area for probe immobilization and reaction, but also a nanopatterned platform for highly efficient bioreactions. In addition, the PEG hydrogel scaffold could decrease the non-specificity adsorption by its anti-adhesive effect, and the decorated aptamer probes in the scaffolds could increase the sensitivity, reliability, and specificity of the bacteria capture and detection. Moreover, the tagged magnetic nanoparticles in the PEG scaffold could impart the barcodes with controllable movement under magnetic fields, which can be used to significantly increase the reaction speed and simplify the processing of the bioassays. Based on the describe barcodes, it was demonstrated that the bacteria could be captured and identified even at low bacterial concentrations (100 CFU mL) within 2.5h, which is effectively shortened in comparison with the "gold standard" in clinic. These features make the barcodes ideal for capturing and detecting multiple bacteria from clinical samples for hematological infection diagnostics.
基于从患者血液中分离和检测病原体来快速、灵敏地诊断血液感染是一个重大挑战。针对这一问题，我们在此提出了一种新的条形码技术，该技术可以从复杂样本中同时捕获和检测多种类型的病原体。条形码是聚乙二醇（PEG）水凝胶反蛋白石颗粒，具有特征反射峰编码，在其表面捕获细菌时保持稳定。由于颗粒的球形表面具有有序的多孔纳米结构，因此条形码不仅可以提供更多的探针固定和反应表面积，还可以提供用于高效生物反应的纳米图案化平台。此外，PEG 水凝胶支架的抗粘附作用可以降低非特异性吸附，支架中修饰的适体探针可以提高细菌捕获和检测的灵敏度、可靠性和特异性。此外，PEG 支架中的标记磁性纳米颗粒可以赋予条形码在磁场下的可控运动能力，这可以显著提高反应速度并简化生物分析的处理过程。基于描述的条形码，证明即使在低细菌浓度（100 CFU mL）下，也可以在 2.5 小时内捕获和鉴定细菌，与临床“金标准”相比，这一过程得到了有效缩短。这些特性使条形码成为从临床样本中捕获和检测多种血液感染病原体的理想选择。
