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超临界流体萃取法去除蛋白核小球藻中的叶绿素和原卟啉:蛋白质资源的潜力。

Removal of chlorophyll and pheophorbide from Chlorella pyrenoidosa by supercritical fluid extraction: potential of protein resource.

机构信息

New Industry Creation Hatchery Center (NICHe), Tohoku University, Aoba-ku, Sendai, Japan.

Biodynamic Pant Institute Co. Ltd, Hokudai Business Spring Bldg, Kita-ku, Sapporo, Hokkaido, Japan.

出版信息

Biosci Biotechnol Biochem. 2021 Jun 24;85(7):1759-1762. doi: 10.1093/bbb/zbab095.

Abstract

This study tried to quantitatively clarify the usefulness of supercritical fluid extraction for removal of chlorophyll and pheophorbide from Chlorella pyrenoidosa. C. pyrenoidosa powder was subjected to supercritical fluid extraction, and chlorophyll a and pheophorbide a in its extracted fractions were measured by HPLC-UV. Chlorophyll a and pheophorbide a in residue after supercritical fluid extraction became below of detection limit.

摘要

本研究试图定量阐明超临界流体萃取在去除蛋白核小球藻中的叶绿素和原卟啉中的有用性。将蛋白核小球藻粉末进行超临界流体萃取,并用 HPLC-UV 测定其萃取部分中的叶绿素 a 和原卟啉 a。超临界流体萃取后残渣中的叶绿素 a 和原卟啉 a 降至检测限以下。

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