Department of Food Science and Engineering, Ocean University of China, Qingdao, 266003, People's Republic of China.
J Sci Food Agric. 2014 Jan 15;94(1):139-45. doi: 10.1002/jsfa.6224. Epub 2013 Jun 17.
The carotenoids and chlorophyll a of Laminaria japonica Aresch were extracted using ethanol-modified subcritical 1,1,1,2-tetrafluoroethane (R134a). In the present study, the effects of pressure (5-17 MPa), temperature (303-333 K) and the amount of cosolvent(2-6% R134a, w/w)were investigated. Response surface methodology (RSM) combined with a Box-Behnken design was applied to evaluate the significance of the three independent variables on each response. A desirability function was conducted to simultaneously optimize the multiple responses.
The optimum extraction conditions were as follows: extraction temperature 324.13 K, extraction pressure 17 MPa and a cosolvent amount of 4.73%. Under these conditions, the yields of carotenoids and chlorophyll a were predicted to be 0.239 and 2.326 g kg(-1), respectively.
It has been proved that subcritical R134a is a potential solvent, which can be an alternative to supercritical CO2 for extraction of natural ingredients under mild conditions.
采用乙醇改性的 1,1,1,2-四氟乙烷（R134a）提取裙带菜的类胡萝卜素和叶绿素 a。本研究考察了压力（5-17 MPa）、温度（303-333 K）和夹带剂用量（2-6% R134a，w/w）的影响。采用响应面法（RSM）结合 Box-Behnken 设计对三个独立变量对每个响应的重要性进行了评价。采用可接受性函数对多个响应进行了同步优化。
最佳提取条件为：提取温度 324.13 K，提取压力 17 MPa，夹带剂用量 4.73%。在此条件下，预测类胡萝卜素和叶绿素 a 的得率分别为 0.239 和 2.326 g kg(-1)。
已证明亚临界 R134a 是一种潜在的溶剂，可在温和条件下替代超临界 CO2 用于天然成分的提取。
