Department of General Surgery, The Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China.
Mol Med Rep. 2021 Jul;24(1). doi: 10.3892/mmr.2021.12167. Epub 2021 May 26.
Gallbladder cancer (GBC) is the most aggressive cancer type in the biliary tract, and our previous studies observed that microRNA (miR)‑135a‑5p expression was downregulated in GBC tissues. However, few studies have focused on the mechanism of action of the miR‑135a‑5p target genes in GBC. The present study aimed to investigate the regulatory role of miR‑135a‑5p signaling in GBC. The present study found that miR‑135a‑5p expression was downregulated in GBC tissue, as detected by immunohistochemistry and reverse transcription‑quantitative PCR. In addition, overexpression of miR‑135a‑5p significantly inhibited the proliferation and migration of GBC‑SD cells. Using a luciferase activity assay, it was identified that angiopoietin‑2 (ANGPT2) was a potential target gene of miR‑135a‑5p in GBC. Knockdown of ANGPT2 expression significantly inhibited the proliferation and invasion of GBC‑SD cells. In conclusion, the present results suggested that miR‑135a‑5p affected GBC cell proliferation and invasion by targeting ANGPT2. Moreover, miR‑135a‑5p may be a potential biomarker for GBC progression and a potential target for GBC therapeutic intervention.
胆囊癌 (GBC) 是胆道系统中最具侵袭性的癌症类型，我们之前的研究观察到 miR-135a-5p 在 GBC 组织中表达下调。然而，很少有研究关注 miR-135a-5p 靶基因在 GBC 中的作用机制。本研究旨在探讨 miR-135a-5p 信号在 GBC 中的调节作用。本研究通过免疫组织化学和逆转录-定量 PCR 检测到 miR-135a-5p 在 GBC 组织中表达下调。此外，miR-135a-5p 的过表达显著抑制 GBC-SD 细胞的增殖和迁移。通过荧光素酶活性测定，鉴定出血管生成素-2 (ANGPT2) 是 GBC 中 miR-135a-5p 的潜在靶基因。ANGPT2 表达的下调显著抑制 GBC-SD 细胞的增殖和侵袭。综上所述，本研究结果表明，miR-135a-5p 通过靶向 ANGPT2 影响 GBC 细胞的增殖和侵袭。此外，miR-135a-5p 可能是 GBC 进展的潜在生物标志物和 GBC 治疗干预的潜在靶点。
