School of Molecular and Life Sciences, Curtin University, Bentley 6102, WA, Australia.
Dipartimento di Scienze Chimiche e Geologiche, Università degli Studi di Modena e Reggio Emilia, via G. Campi 103, 41125 Modena, Italy.
Analyst. 2021 Jun 14;146(12):3818-3822. doi: 10.1039/d1an00363a.
There is a lack of molecular probes for imaging bacteria, in comparison to the array of such tools available for the imaging of mammalian cells. Here, organometallic molecular probes have been developed and assessed for bacterial imaging, designed to have the potential to support multiple imaging modalities. The chemical structure of the probes is designed around a metal-naphthalimide structure. The 4-amino-1,8-naphthalimide moiety, covalently appended through a pyridine ancillary ligand, acts as a luminescent probe for super-resolution microscopy. On the other hand, the metal centre, rhenium(i) or platinum(ii) in the current study, enables techniques such as nanoSIMS. While the rhenium(i) complex was not sufficiently stable to be used as a probe, the platinum(ii) analogue showed good chemical and biological stability. Structured illumination microscopy (SIM) imaging on live Bacillus cereus confirmed the suitability of the probe for super-resolution microscopy. NanoSIMS analysis was used to monitor the uptake of the platinum(ii) complex within the bacteria and demonstrate the potential of this chemical architecture to enable multimodal imaging. The successful combination of these two moieties introduces a platform that could lead to a versatile range of multi-functional probes for bacteria.
与用于哺乳动物细胞成像的众多工具相比，用于成像细菌的分子探针还比较缺乏。在此，开发了用于细菌成像的有机金属分子探针，并对其进行了评估，这些探针具有支持多种成像模式的潜力。探针的化学结构设计围绕金属-萘酰亚胺结构。通过吡啶辅助配体共价连接的 4-氨基-1,8-萘酰亚胺部分充当超分辨率显微镜的荧光探针。另一方面，金属中心（本研究中为铼(i)或铂(ii)）使纳米二次离子质谱（nanoSIMS）等技术成为可能。虽然铼(i)配合物不够稳定，无法用作探针，但铂(ii)类似物表现出良好的化学和生物稳定性。对活的蜡状芽孢杆菌进行结构光照明显微镜（SIM）成像证实了该探针适用于超分辨率显微镜。纳米二次离子质谱（nanoSIMS）分析用于监测铂(ii)配合物在细菌内的摄取情况，并证明了这种化学结构在实现多模态成像方面的潜力。这两种基团的成功结合引入了一个平台，该平台可能会导致一系列用于细菌的多功能多功能探针。
