Guo Ting, Wang Changchang, Zhou Hongyuan, Zhang Yuhao, Ma Liang, Wang Shuo
Ministry of Education, College of Food Science, Southwest University, Chongqing 400715, P. R. China.
Institute of Environment and Safety, Wuhan Academy of Agricultural Science, Wuhan 430207, P. R. China.
Anal Methods. 2021 Jun 24;13(24):2654-2658. doi: 10.1039/d1ay00642h.
A facile fluorescent aptasensor based on polydopamine nanospheres (PDANSs) has been proposed for the rapid and high sensitive sensing of T-2 toxin. PDANSs are dopamine-derived synthetic eumelanin polymers with excellent fluorescence quenching ability, dispersibility and biocompatibility. In the assay, 6-carboxyfluorescein (FAM)-labeled aptamers (FAM-aptamers) were adsorbed onto PDANSs via noncovalent bonding, resulting in quenching fluorescence. In the presence of T-2, the binding of T-2 to the aptamers could promote the formation of the A-form duplex hairpin structure, which was used as a sensing platform to detect T-2 on the basis of fluorescence recovery. The results showed that the aptasensor was rapid and sensitive for the detection of T-2 toxin with a linear detection range of 10-180 μg L-1 and a detection limit of 7.23 μg L-1. The performance of the proposed method was comparable with that of the liquid chromatography-mass spectrometry method (LC-MS). Thus, the aptasensor could be used for the determination of real samples. The design method proposed in this study provides a strategy for the development of PDANS-based toxin biosensors.
一种基于聚多巴胺纳米球（PDANSs）的简易荧光适配体传感器被用于快速、高灵敏地检测T-2毒素。PDANSs是由多巴胺衍生而来的合成真黑素聚合物，具有出色的荧光猝灭能力、分散性和生物相容性。在该检测方法中，6-羧基荧光素（FAM）标记的适配体（FAM-适配体）通过非共价键吸附在PDANSs上，导致荧光猝灭。在存在T-2的情况下，T-2与适配体的结合可促进A-型双链发夹结构的形成，该结构被用作基于荧光恢复检测T-2的传感平台。结果表明，该适配体传感器对T-2毒素的检测快速且灵敏，线性检测范围为10 - 180 μg L-1，检测限为7.23 μg L-1。该方法的性能与液相色谱-质谱法（LC-MS）相当。因此，该适配体传感器可用于实际样品的测定。本研究提出的设计方法为基于PDANSs的毒素生物传感器的开发提供了一种策略。
