Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, PR China.
Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, PR China; School of Public Health, Lanzhou University, Lanzhou, 730030, PR China.
Anal Chim Acta. 2021 May 22;1160:338450. doi: 10.1016/j.aca.2021.338450. Epub 2021 Mar 26.
T-2 toxin is a class A trichothecene mycotoxin produced by Fusarium, which exhibits genotoxic, cytotoxic, and immunotoxic effects in animals and humans. In this study, we developed an aptasensor for the sensitive detection of T-2 toxin, which was based on fluorescence resonance energy transfer (FRET), and acted by adjusting the electric potential on the surface of upconversion nanoparticles (UCNPs) and MIL-101(Cr). In addition, it combined the excellent spectral properties of UCNPs with the good adsorption quenching abilities of metal organic frameworks (MOFs). Under the action of π-π stacking interactions, the UCNPs-aptamer was adsorbed onto the surface of MIL-101, leading to fluorescence quenching due to the occurrence of FRET. After the addition of T-2 toxin, owing to its selective binding to the UCNPs-aptamer, the UCNPs-aptamer moved away from MIL-101(Cr), resulting in fluorescence recovery. Moreover, the extent of fluorescence recovery was positively correlated with the concentration of T-2 toxin. The limit of detection (LOD) of this sensor was 0.087 ng mL (S/N = 3), and a good linear correlation was observed between the fluorescence intensity and the T-2 toxin concentration in the range of 0.1-100 ng mL. Moreover, the recovery of this method was 97.52-109.53% for corn meal samples (relative standard deviation, RSD = 1.7-2.4%) and 90.81-100.02% for beer samples (RSD = 2.4-2.7%). By adjusting the surface electric potentials, the efficient fluorescence aptasensor combined the advantages of UCNPs and MIL-101(Cr) and allowed the first application of such a system in toxin detection, thereby indicating its potential food sample analysis and biochemical sensing.
T-2 毒素是一种由镰刀菌产生的 A 类单端孢霉烯族真菌毒素，在动物和人类中具有遗传毒性、细胞毒性和免疫毒性作用。在本研究中，我们开发了一种基于荧光共振能量转移（FRET）的 T-2 毒素灵敏检测的适体传感器，该传感器通过调节上转换纳米粒子（UCNPs）和 MIL-101（Cr）表面的电势来工作。此外，它结合了 UCNPs 的优异光谱特性和金属有机骨架（MOFs）的良好吸附猝灭能力。在π-π 堆积相互作用的作用下，UCNPs-适体被吸附到 MIL-101 的表面，由于 FRET 的发生导致荧光猝灭。加入 T-2 毒素后，由于其与 UCNPs-适体的选择性结合，UCNPs-适体从 MIL-101（Cr）上移动，导致荧光恢复。此外，荧光恢复的程度与 T-2 毒素的浓度呈正相关。该传感器的检测限（LOD）为 0.087ngmL（S/N=3），在 0.1-100ngmL 范围内荧光强度与 T-2 毒素浓度之间呈现良好的线性关系。此外，对于玉米粉样品，该方法的回收率为 97.52-109.53%（相对标准偏差，RSD=1.7-2.4%），对于啤酒样品，回收率为 90.81-100.02%（RSD=2.4-2.7%）。通过调节表面电势，高效荧光适体传感器结合了 UCNPs 和 MIL-101（Cr）的优势，并首次将该系统应用于毒素检测，从而表明其在食品样品分析和生化传感方面具有应用潜力。
