Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, PR China.
Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, PR China; School of Public Health, Lanzhou University. Lanzhou 730030, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Oct 5;259:119893. doi: 10.1016/j.saa.2021.119893. Epub 2021 May 4.
T-2 toxin is one of class A trichothecene mycotoxins produced by Fusarium, presenting genotoxic, cytotoxicity, and immunotoxicity for animals and humans. Therefore, It is urgent to establish a rapid test method with high sensitivity, good selectivity and reliability. In this research, by adjusting the synthesis conditions, a kind of NH-UiO-66 with high quenching efficiency was screened out. On this basis, we constructed a novel fluorescence sensor via Cy3-labeled aptamer (Cy3-aptamer). With the help of π-π interaction, hydrogen bond and coordination, NH-UiO-66 could adsorb and quench the fluorescence of Cy3-aptamer based on FRET and PET. In the presence of T-2 toxin, it recognized and bound to Cy3-aptamer, leading to the disintegration of the NH-UiO-66/Cy3-aptamer compound. As the energy transfer process was blocked, the fluorescence intensity was restored, enabling a highly sensitive response to T-2 toxin. There was a good linear correlation between fluorescence intensity and T-2 toxin concentration in the range of 0.5-100 ng ml . The LOD of this fluorescence aptasensor was 0.239 ng ml (S/N = 3). Besides, the recoveries of milk and beer were 89.86-108.99% (RSD = 2.0-2.6%) and 92.31-111.51% (RSD = 2.3-2.9%), respectively. The fluorescence aptasensor exhibited advantages of excellent analytical performance, convenient operation procedure and good selectivity. Predictably, the aptasensor was supposed to detect antibiotics and other pollutants, describing an intriguing blueprint and potential application prospect in food safety, biochemical sensing and environmental conservation.
T-2 毒素是镰刀菌产生的 A 类单端孢霉烯族真菌毒素之一，对动物和人类具有遗传毒性、细胞毒性和免疫毒性。因此，迫切需要建立一种灵敏度高、选择性好、可靠性强的快速检测方法。本研究通过调整合成条件，筛选出一种具有高猝灭效率的 NH-UiO-66。在此基础上，我们通过 Cy3 标记的适体（Cy3-aptamer）构建了一种新型荧光传感器。在π-π 相互作用、氢键和配位作用的帮助下，NH-UiO-66 可以吸附和猝灭基于 FRET 和 PET 的 Cy3-aptamer 的荧光。在 T-2 毒素存在下，它识别并与 Cy3-aptamer 结合，导致 NH-UiO-66/Cy3-aptamer 化合物解体。由于能量转移过程被阻断，荧光强度得到恢复，对 T-2 毒素产生了高灵敏度的响应。荧光强度与 T-2 毒素浓度在 0.5-100ng/ml 范围内呈良好的线性关系。该荧光适体传感器的检出限为 0.239ng/ml（S/N=3）。此外，牛奶和啤酒的回收率分别为 89.86-108.99%（RSD=2.0-2.6%）和 92.31-111.51%（RSD=2.3-2.9%）。该荧光适体传感器具有分析性能优异、操作简便、选择性好等优点。可以预见，该适体传感器有望用于检测抗生素等污染物，为食品安全、生化传感和环境保护描绘了一个引人入胜的蓝图和潜在的应用前景。
