Silva I D, Jain N C
Department of Clinical Pathology, School of Veterinary Medicine, University of California, Davis 95616.
J Dairy Sci. 1988 Jun;71(6):1625-31. doi: 10.3168/jds.S0022-0302(88)79726-X.
A comparative evaluation of in vitro phagocytic and metabolic activities of freshly isolated bovine blood neutrophils from 26 cows was performed. Degrees of phagocytosis and nitroblue tetrazolium reduction by neutrophils were measured using zymosan, Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae, Mycoplasma bovis, Salmonella sp., and Brucella abortus opsonized with fresh bovine serum. Percent phagocytosis and nitroblue tetrazolium reduction by the neutrophils were determined by microscopic evaluation of stained coverslip smears. Individual variation among cows was found in parameters measured, but age was not a contributing factor. Phagocytosis was high (73 to 81%) for E. coli, Salmonella sp., Strep. agalactiae, and zymosan; intermediate (64%) for Staph. aureus; and low (24 to 40%) for M. bovis and B. abortus. Nitroblue tetrazolium reduction was distinctly lower than zymosan (74%) for all microorganisms. Similar percentages (32 to 41%) of nitroblue tetrazolium reduction were observed for E. coli, Staph. aureus, Strep. agalactiae, and M. bovis; intermediate (28%) was obtained for Salmonella sp. and lowest (11%) for B. abortus.
对来自26头奶牛的新鲜分离的牛血中性粒细胞的体外吞噬和代谢活性进行了比较评估。使用经新鲜牛血清调理的酵母聚糖、大肠杆菌、金黄色葡萄球菌、无乳链球菌、牛支原体、沙门氏菌属和流产布鲁氏菌来测量中性粒细胞的吞噬程度和硝基蓝四氮唑还原能力。通过对染色盖玻片涂片的显微镜评估来确定中性粒细胞的吞噬百分比和硝基蓝四氮唑还原能力。在所测量的参数中发现奶牛之间存在个体差异，但年龄不是一个影响因素。大肠杆菌、沙门氏菌属、无乳链球菌和酵母聚糖的吞噬率较高（73%至81%）；金黄色葡萄球菌的吞噬率中等（64%）；牛支原体和流产布鲁氏菌的吞噬率较低（24%至40%）。所有微生物的硝基蓝四氮唑还原能力明显低于酵母聚糖（74%）。大肠杆菌、金黄色葡萄球菌、无乳链球菌和牛支原体的硝基蓝四氮唑还原百分比相似（32%至41%）；沙门氏菌属的还原率中等（28%），流产布鲁氏菌的还原率最低（11%）。
