Department of Radiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Department of Radiology, Daping Hospital, Army Medical University, Chongqing, 400016, China.
Sci Rep. 2021 May 26;11(1):11060. doi: 10.1038/s41598-021-90597-6.
The aim of this study was to investigate the mechanisms underlying demyelination and remyelination with 7.0 T multiparameter magnetic resonance imaging (MRI) in an alternative cuprizone (CPZ) mouse model of multiple sclerosis (MS). Sixty mice were divided into six groups (n = 10, each), and these groups were imaged with 7.0 T multiparameter MRI and treated with an alternative CPZ administration schedule. T-weighted imaging (TWI), susceptibility-weighted imaging (SWI), and diffusion tensor imaging (DTI) were used to compare the splenium of the corpus callosum (sCC) among the groups. Prussian blue and Luxol fast blue staining were performed to assess pathology. The correlations of the mean grayscale value (mGSV) of the pathology results and the MRI metrics were analyzed to evaluate the multiparameter MRI results. One-way ANOVA and post hoc comparison showed that the normalized TWI (T-nor), fractional anisotropy (FA), mean diffusivity (MD), radial diffusivity (RD), and axial diffusivity (AD) values were significantly different among the six groups, while the mean phase (Φ) value of SWI was not significantly different among the groups. Correlation analysis showed that the correlation between the T-nor and mGSV was higher than that among the other values. The correlations among the FA, RD, MD, and mGSV remained instructive. In conclusion, ultrahigh-field multiparameter MRI can reflect the pathological changes associated with and the underlying mechanisms of demyelination and remyelination in MS after the successful establishment of an acute CPZ-induced model.
本研究旨在利用 7.0T 多参数磁共振成像（MRI）探讨脱髓鞘和髓鞘再生的机制，并建立多发性硬化症（MS）的替代杯状醇（CPZ）小鼠模型。将 60 只小鼠分为 6 组（每组 n=10），并对这些组进行 7.0T 多参数 MRI 成像和替代 CPZ 给药方案治疗。使用 T 加权成像（TWI）、磁化率加权成像（SWI）和弥散张量成像（DTI）来比较胼胝体压部（sCC）的各组之间的差异。普鲁士蓝和卢索快蓝染色用于评估病理学。分析病理学结果和 MRI 指标的平均灰度值（mGSV）之间的相关性，以评估多参数 MRI 结果。单因素方差分析和事后比较显示，归一化 TWI（T-nor）、各向异性分数（FA）、平均弥散度（MD）、径向弥散度（RD）和轴向弥散度（AD）值在 6 组之间存在显著差异，而 SWI 的平均相位（Φ）值在组间无显著差异。相关性分析表明，T-nor 与 mGSV 的相关性高于其他值。FA、RD、MD 和 mGSV 之间的相关性仍然具有指示意义。总之，超高场多参数 MRI 可以反映急性 CPZ 诱导模型建立后 MS 脱髓鞘和髓鞘再生相关的病理变化及其潜在机制。
