用于复杂培养物确认的侧向流动分析的效用。
Utility of a lateral flow assay for culture confirmation of complex.
作者信息
Kumar Mahadevan, Handique Firuj
机构信息
Associate Professor, Dept of Microbiology, Armed Forces Medical College, Pune 411040, India.
Resident, Dept of Microbiology, Armed Forces Medical College, Pune 411040, India.
出版信息
Med J Armed Forces India. 2016 Jan;72(1):41-44. doi: 10.1016/j.mjafi.2014.12.021. Epub 2015 Feb 25.
BACKGROUND
Therapy for the clinical management of patients with Mycobacterium tuberculosis complex (MTBC) and non tuberculous mycobacteria (NTM) is different. Prompt detection and discrimination is necessary for administration of suitable therapy.
METHODS
The aim of the study was to evaluate the performance of a Immunochromatographic Test (ICT) in rapid differentiation of MTBC from NTM grown in Lowenstein-Jensen medium and MGIT broth in comparison to molecular methods.
RESULTS
Of the 106 isolates in this study, 96 and 95 were identified as MTBC by 16S rRNA PCR and MPT64 respectively. The sensitivity and specificity of MPT64 was found to be 99% and 100% respectively.
CONCLUSION
The Lateral Flow Assay Test is a useful and specific tool in rapid differentiation of M. tuberculosis complex from culture. Therefore proper identification avoids unnecessary ATT to patients infected with NTM.
背景
结核分枝杆菌复合群（MTBC）患者与非结核分枝杆菌（NTM）患者的临床管理治疗方法不同。为了进行适当的治疗，需要及时检测和鉴别。
方法
本研究的目的是评估免疫层析试验（ICT）与分子方法相比，在快速区分在罗-琴培养基和分枝杆菌生长指示管肉汤中生长的MTBC和NTM方面的性能。
结果
在本研究的106株分离株中，分别有96株和95株通过16S rRNA PCR和MPT64鉴定为MTBC。发现MPT64的敏感性和特异性分别为99%和100%。
结论
侧向流动分析试验是从培养物中快速区分结核分枝杆菌复合群的一种有用且特异的工具。因此，正确的鉴定可避免对感染NTM的患者进行不必要的抗结核治疗。
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