Tasaka H, Shigeto E, Matsuo K, Yamaguchi R, Haga S, Yamazaki A, Yamazaki T, Nagai S, Nakamura R M
Department of Bacteriology, Hiroshima University School of Medicine, Japan.
Scand J Immunol. 1995 Oct;42(4):487-92. doi: 10.1111/j.1365-3083.1995.tb03684.x.
MPB64, a specific antigen to Mycobacterium tuberculosis complex (TB complex), was produced and secreted by a clone of M. smegmatis-MPB64 where the structural gene of MPB64 was inserted using a new mycobacteria, E. coli shuttle plasmid pNIS vector. Antibodies against the recombinant MPB64 (rMPB64) were used for the reverse particle latex agglutination (RPLA) test to detect the MPB64 antigen rapidly. RPLA tests were applied to the shock cultures and the clinical isolates of mycobacteria to identify TB complex. RPLA with anti-MPB64 antibody-coated latex beads completely distinguished TB complex from other mycobacteria. Thus, it is suggested that RPLA with anti-MPB64 antibody would be a new, easy and inexpensive method for the rapid diagnosis of tuberculosis.
MPB64是结核分枝杆菌复合群（结核菌复合群）的一种特异性抗原，由耻垢分枝杆菌-MPB64克隆株产生并分泌，该克隆株使用新型分枝杆菌-大肠杆菌穿梭质粒pNIS载体插入了MPB64的结构基因。针对重组MPB64（rMPB64）的抗体用于反向颗粒乳胶凝集（RPLA）试验，以快速检测MPB64抗原。RPLA试验应用于分枝杆菌的震荡培养物和临床分离株，以鉴定结核菌复合群。用抗MPB64抗体包被的乳胶珠进行的RPLA试验能将结核菌复合群与其他分枝杆菌完全区分开来。因此，提示用抗MPB64抗体进行RPLA试验将是一种用于快速诊断结核病的新的、简便且廉价的方法。
