Wu Yuanbo, Zhang Jiandong, Ni Jiangwei, Yang Zhihao, Chen Kun, Zheng Liangcheng, He Zhifeng
Department of Cardiothoracic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.
Department of Thoracic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.
Cancer Manag Res. 2021 May 18;13:4067-4076. doi: 10.2147/CMAR.S291473. eCollection 2021.
This study aimed to evaluate the properties and functions of polysaccharide-based porous microsphere (PPM) for drug delivery, as well as its inhibitory effect on malignant tumors.
PPM was prepared using the inverse emulsion polymerization method. FT-IR measurements were conducted to measure the wavenumber of PPM. Particle size distribution was tested with a particle analyzer, and surface morphologies of PPM were observed using a scanning electron microscope (SEM). Dialysis method, Cell Counting Kit-8 (CCK-8), and cell apoptosis analysis were adopted to evaluate the drug release, cytotoxicity and biocompatibility of mitomycin-C (MMC), respectively. Finally, an in vivo study was performed in C57BL/6 mice to confirm the function of MMC-loaded PPM on tumor growth.
FT-IR spectra proved the successful preparation of MMC-loaded PPM. PPM had an average size of 25.90 ± 0.34 μm and then increased to 30.10 ± 0.20 μm after drug loading. Under SEM, the surface morphology was lotus seedpod surface-like, with macropits on the surface and micropores in macropits. Compared with the free MMC group, MMC-loaded PPM exhibited a delayed drug release rate in a pH-dependent manner and higher cell viability. Flow cytometry results showed that the cell apoptosis in the PPM/MMC group was lower than that in the free MMC group. In vivo experiment revealed the inhibitory efficacy of MMC-loaded PPM on malignant tumors.
In summary, MMC-loaded PPM exhibited favorable surface morphology, sustained drug release ability, nontoxicity and excellent biocompatibility, suggesting that PPM might be a potential drug carrier for tumor treatment.
本研究旨在评估基于多糖的多孔微球（PPM）用于药物递送的性质和功能，以及其对恶性肿瘤的抑制作用。
采用反相乳液聚合法制备PPM。进行傅里叶变换红外光谱（FT-IR）测量以测定PPM的波数。用颗粒分析仪测试粒径分布，并用扫描电子显微镜（SEM）观察PPM的表面形态。分别采用透析法、细胞计数试剂盒-8（CCK-8）和细胞凋亡分析来评估丝裂霉素-C（MMC）的药物释放、细胞毒性和生物相容性。最后，在C57BL/6小鼠中进行体内研究，以确认载有MMC的PPM对肿瘤生长的作用。
FT-IR光谱证明成功制备了载有MMC的PPM。PPM的平均粒径为25.90±0.34μm，载药后增至30.10±0.20μm。在SEM下，表面形态呈莲蓬表面状，表面有大凹坑，大凹坑内有微孔。与游离MMC组相比，载有MMC的PPM表现出pH依赖性的药物释放速率延迟和更高的细胞活力。流式细胞术结果显示，PPM/MMC组的细胞凋亡低于游离MMC组。体内实验揭示了载有MMC的PPM对恶性肿瘤的抑制效果。
总之，载有MMC的PPM表现出良好的表面形态、持续的药物释放能力、无毒性和优异的生物相容性，表明PPM可能是一种用于肿瘤治疗的潜在药物载体。
