Zhao Chuanyi, Wei Mengyuan, Zheng Yilin, Tao Weili, Lv Qian, Wang Qiongjin, Wang Shuyun, Chen Yicun
Department of Pharmacology, Shantou University Medical College, Shantou University, Shantou, China.
Front Pharmacol. 2021 May 10;12:624296. doi: 10.3389/fphar.2021.624296. eCollection 2021.
(DC.) Hand.-Mazz (OH), also known as sweet tea, is a valuable functional food with medicinal properties and is used for the treatment of cold, cough, gastroenteritis, heatstroke, herpes zoster, and rheumatoid arthritis. The phytochemicals in plant-based foods are responsible for the occurrence of these diseases to some extent. However, there is a scarcity of information on the chemical components of OH. We, therefore, aimed to investigate the phytochemical components of OH using ultra high-performance liquid chromatography-mass spectrometry (UHPLC-MS) and UHPLC triple time-of-flight mass spectrometry (UHPLC-Triple-TOF-MS). The main component of the OH extract, asperulosidic acid, was additionally quantified using UHPLC with ultraviolet detection (UHPLC-UV). The anticancer activity of the OH extract was assessed by a cell proliferation assay and a scratch assay using an esophageal cancer cell line. Ten compounds were tentatively identified in the aqueous extract of OH, including five iridoids, two anthraquinones, and one phenolic acid. The content of asperulosidic acid in the aqueous extract of OH was approximately 42 μg ml, and the extract exerted definite anticancer effects. The results can be used for quality control and assessment of the OH extract, which can serve as a promising source of functional ingredients for potential use in the food and drug industries.
（DC.）Hand.-Mazz（OH），也被称为甜茶，是一种具有药用特性的珍贵功能性食品，用于治疗感冒、咳嗽、肠胃炎、中暑、带状疱疹和类风湿性关节炎。植物性食物中的植物化学物质在一定程度上导致了这些疾病的发生。然而，关于OH化学成分的信息却很匮乏。因此，我们旨在使用超高效液相色谱-质谱联用仪（UHPLC-MS）和超高效液相色谱三重四极杆飞行时间质谱仪（UHPLC-Triple-TOF-MS）来研究OH的植物化学成分。OH提取物的主要成分车叶草苷酸，另外还使用带紫外检测的超高效液相色谱（UHPLC-UV）进行了定量分析。通过使用食管癌细胞系的细胞增殖试验和划痕试验评估了OH提取物的抗癌活性。在OH的水提取物中初步鉴定出了10种化合物，包括5种环烯醚萜、2种蒽醌和1种酚酸。OH水提取物中车叶草苷酸的含量约为42μg/ml，且该提取物具有一定的抗癌作用。这些结果可用于OH提取物的质量控制和评估，OH提取物有望成为食品和制药行业潜在使用的功能性成分的来源。
