Department of Microbiology, Osmania University, Hyderabad, Telangana, India.
Department of Microbiology, PSGVP Mandal's Arts, Science and Commerce College, Shahada, Maharashtra, India.
PLoS One. 2021 Mar 18;16(3):e0241729. doi: 10.1371/journal.pone.0241729. eCollection 2021.
Silver nanoparticles (AgNPs) are among the most widely synthesized and used nanoparticles (NPs). AgNPs have been traditionally synthesized from plant extracts, cobwebs, microorganisms, etc. However, their synthesis from wing extracts of common insect; Mang mao which is abundantly available in most of the Asian countries has not been explored yet. We report the synthesis of AgNPs from M. mao wings extract and its antioxidant and antimicrobial activity. The synthesized AgNPs were spherical, 40-60 nm in size and revealed strong absorption plasmon band around at 430 nm. Highly crystalline nature of these particles as determined by Energy-dispersive X-ray analysis and X-ray diffraction further confirmed the presence of AgNPs. Hydrodynamic size and zeta potential of AgNPs were observed to be 43.9 nm and -7.12 mV, respectively. Fourier-transform infrared spectroscopy analysis revealed the presence of characteristic amide proteins and aromatic functional groups. Thin-layer chromatography (TLC) and Gas chromatography-mass spectroscopy (GC-MS) analysis revealed the presence of fatty acids in the wings extract that may be responsible for biosynthesis and stabilization of AgNPs. Further, SDS-PAGE of the insect wing extract protein showed the molecular weight of 49 kDa. M. mao silver nanoparticles (MMAgNPs) exhibit strong antioxidant, broad-range antibacterial and antifungal activities, (66.8 to 87.0%), broad-range antibacterial and antifungal activities was found with maximum zone of inhibition against Staphylococcus aureus MTCC 96 (35±0.4 mm) and Fusarium oxysporum f. sp. ricini (86.6±0.4) which signifies their biomedical and agricultural potential.
银纳米粒子（AgNPs）是最广泛合成和使用的纳米粒子（NPs）之一。AgNPs 传统上是从植物提取物、蜘蛛网、微生物等中合成的。然而，从常见昆虫的翅膀提取物中合成 AgNPs 尚未得到探索；在大多数亚洲国家都有大量的毛蚊，这为 AgNPs 的合成提供了丰富的原料。我们报告了从毛蚊翅膀提取物中合成 AgNPs 及其抗氧化和抗菌活性。合成的 AgNPs 呈球形，尺寸为 40-60nm，并在 430nm 左右显示出强烈的吸收等离子体带。通过能量色散 X 射线分析和 X 射线衍射确定这些颗粒具有高度结晶性，进一步证实了 AgNPs 的存在。AgNPs 的水动力尺寸和 zeta 电位分别观察到为 43.9nm 和-7.12mV。傅里叶变换红外光谱分析显示存在特征酰胺蛋白和芳香族官能团。薄层层析（TLC）和气相色谱-质谱（GC-MS）分析表明，翅膀提取物中存在脂肪酸，可能负责 AgNPs 的生物合成和稳定。此外，昆虫翅膀提取物蛋白的 SDS-PAGE 显示分子量为 49kDa。毛蚊银纳米粒子（MMAgNPs）表现出强烈的抗氧化、广谱抗菌和抗真菌活性（66.8 至 87.0%），对金黄色葡萄球菌 MTCC 96（35±0.4mm）和立枯丝核菌（Fusarium oxysporum f.sp.ricini）具有最大抑菌圈（86.6±0.4），这表明它们具有生物医学和农业潜力。
