Li Binjie, Zhao Huimin, Yu Mingjia
Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, China.
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
Int J Anal Chem. 2021 May 6;2021:5543460. doi: 10.1155/2021/5543460. eCollection 2021.
Heparins and sulfated polysaccharides have been recognized as effective clinical anticoagulants for several decades. Heparins exhibit heterogeneity depending on the sources. Meanwhile, the adverse effect in the clinical uses and the adulteration of oversulfated chondroitin sulfate (OSCS) in heparins develop additional attention to analyze the purity of heparins. This review starts with the description of the classification, anticoagulant mechanism, clinical application of heparins and focuses on the existing methods of heparin analysis and detection including traditional detection methods, as well as new methods using fluorescence or gold nanomaterials as probes. The in-depth understanding of these techniques for the analysis of heparins will lay a foundation for the further development of novel methods for the detection of heparins.
几十年来，肝素和硫酸化多糖一直被认为是有效的临床抗凝剂。肝素根据来源表现出异质性。同时，肝素临床使用中的不良反应以及硫酸软骨素（OSCS）在肝素中的掺假问题，使得人们更加关注分析肝素的纯度。本综述首先描述了肝素的分类、抗凝机制、临床应用，并重点介绍了现有的肝素分析和检测方法，包括传统检测方法以及使用荧光或金纳米材料作为探针的新方法。深入了解这些肝素分析技术将为进一步开发新型肝素检测方法奠定基础。
