College of Physics, Dalian University of Technology, Dalian, 116024, China.
School of Chemical Engineering, Dalian University of Technology, Dalian, 116024, China.
Biosens Bioelectron. 2020 Apr 15;154:112039. doi: 10.1016/j.bios.2020.112039. Epub 2020 Jan 22.
A label-free Au NPs-enhanced surface plasmon resonance (SPR) sensor was developed for the ultrasensitive detection of heparin based on competitive adsorption behavior of heparin and Au NPs on the poly (dimethyl-diallylammonium chloride) (PDDA)-modified optical fiber surface and the corresponding change in the resonance wavelength of SPR. Due to the high affinity between heparin and PDDA, the present senor shows good analytical performance with respect to heparin detection. Two obvious advantages of the proposed heparin sensor over other reported methods are: its much wider linear concentration range (10-10 g/mL) and lower limit of detection (0.0257 ng/mL). The analysis of heparin in serum demonstrated that the present sensor exhibited high sensitivity and selectivity. It should be noted that the sensing strategy takes advantage of a portable fiber-optic SPR sensing system and avoids the need for complex processes for labeled-Au NPs, and thus the present sensor promises to be a practical tool for the point-of-care monitoring of heparin.
一种无标记的金纳米粒子增强表面等离子体共振（SPR）传感器被开发出来，用于基于肝素和金纳米粒子在聚二甲基二烯丙基氯化铵（PDDA）修饰的光纤表面上的竞争吸附行为以及 SPR 的共振波长的相应变化，对肝素进行超灵敏检测。由于肝素和 PDDA 之间具有很高的亲和力，因此本传感器在肝素检测方面表现出良好的分析性能。与其他报道的方法相比，该肝素传感器具有两个明显的优势：其线性浓度范围（10-10 g/mL）更宽，检测下限（0.0257 ng/mL）更低。对血清中肝素的分析表明，本传感器具有高灵敏度和选择性。值得注意的是，该传感策略利用了便携式光纤 SPR 传感系统，并且避免了对标记的 Au NPs 的复杂处理，因此本传感器有望成为即时检测肝素的实用工具。
