Dhir Sunny, Mathioudakis Matthaios M, Hasiów-Jaroszewska Beata, Hallan Vipin
Plant Virus Lab, Council of Scientific & Industrial Research, Institute of Himalayan Bioresource Technology, Palampur, HP 176061 India.
Amity Institute of Virology and Immunology, Amity University Uttar Pradesh, Noida, Uttar Pradesh 201313 India.
3 Biotech. 2021 Jun;11(6):278. doi: 10.1007/s13205-021-02798-5. Epub 2021 May 19.
Recombination leads to the generation of new viral progeny which remain undetected by routine testing procedures and may be a threat to the infected host. Here, we have characterised the complete genome sequences of two isolates of from apple cv. Red Chief (Palampur) and cv. Gold Spur (N) with distinct serological reactivities. The viral genomes consisted of 9267 nucleotides for isolate Palampur and 9254 nucleotides for isolate N, excluding the poly (A) tail and contained 5five open reading frames (ORFs). Isolate N shared 80.8% sequence identity with ASPV apple isolate GA2 from China, while isolate Palampur shared 81.4% sequence identity with ASPV apple isolate PB66 from the United Kingdom. The serological difference of isolates N and Palampur along with their low sequence identity indicated the existence of two distinct virus genotypes which was corroborated by evolutionary and genetic differentiation analyses. Recombination events were detected in the RdRp and CP sequences of Palampur isolate thereby suggesting the role of recombination in the evolution of distinct virus genotypes.
The online version contains supplementary material available at 10.1007/s13205-021-02798-5.
重组导致产生新的病毒后代，这些后代通过常规检测程序无法检测到，并且可能对受感染宿主构成威胁。在这里，我们对来自苹果品种红元帅（帕拉姆布尔）和金冠（N）的两个分离株的完整基因组序列进行了表征，它们具有不同的血清学反应性。病毒基因组中，帕拉姆布尔分离株由9267个核苷酸组成，N分离株由9254个核苷酸组成，不包括聚（A）尾，并且包含5个开放阅读框（ORF）。N分离株与来自中国的苹果褪绿叶斑病毒（ASPV）分离株GA2的序列同一性为80.8%，而帕拉姆布尔分离株与来自英国的ASPV苹果分离株PB66的序列同一性为81.4%。N和帕拉姆布尔分离株的血清学差异以及它们较低的序列同一性表明存在两种不同的病毒基因型，进化和遗传分化分析证实了这一点。在帕拉姆布尔分离株的RNA依赖的RNA聚合酶（RdRp）和外壳蛋白（CP）序列中检测到重组事件，从而表明重组在不同病毒基因型进化中的作用。
在线版本包含可在10.1007/s13205-021-02798-5获取的补充材料。
