Brakta Ajay, Handa Anil, Thakur P D, Tomar Manica, Kumar Pardeep
Plant Virology Laboratory, Department of Plant Pathology, College of Horticulture, Dr. Y.S. Parmar University of Horticulture & Forestry, Nauni-Solan, 173230 HP India.
Virusdisease. 2015 Jun;26(1-2):92-6. doi: 10.1007/s13337-014-0242-8. Epub 2015 Feb 8.
Apple stem pitting foveavirus (ASPV) is one of the most important and widespread virus infecting apples in the world. Of late, the virus has been found to be invariably associated with most of the apple plantations of Shimla district of Himachal Pradesh based on DAS-ELISA results. Bioassay of viruses in vegetatively propagated crops including apple is considered to be an essential component in indexing programmes for the production of virus free propagating material. Woody indicator Malus pumila 'Spy 227' was used for the detection of ASPV through double grafting method. Graft incompatibility and epinasty symptoms were observed on Malus pumila Spy 227 indicator plants. Further, molecular identification of the virus isolate was done by cloning and sequencing of the test isolate. Partial sequence analysis of the coat protein gene showed 89 % nucleotide identity in BLASTN analysis with ASPV isolate from China (Accession No. JF895517). This is the first record of ASPV producing Graft incompatibility on Spy 227 indicator plants.
苹果茎痘凹痕病毒（ASPV）是世界上感染苹果的最重要且分布最广的病毒之一。最近，基于双抗夹心酶联免疫吸附测定（DAS-ELISA）结果发现，该病毒始终与喜马偕尔邦西姆拉地区的大多数苹果种植园有关。在包括苹果在内的无性繁殖作物中进行病毒生物测定，被认为是生产无病毒繁殖材料的检测程序中的一个重要组成部分。木本指示植物苹果品种“斯派227”被用于通过双重嫁接法检测ASPV。在苹果品种斯派227指示植物上观察到了嫁接不亲和性和叶片向下弯曲症状。此外，通过对测试分离株进行克隆和测序，对该病毒分离株进行了分子鉴定。外壳蛋白基因的部分序列分析显示，在BLASTN分析中，与来自中国的ASPV分离株（登录号JF895517）有89%的核苷酸同一性。这是ASPV在斯派227指示植物上产生嫁接不亲和性的首次记录。
