Murakami T, Matsuyama T
Department of Virology, Osaka City Institute of Public Health and Environmental Sciences, Japan.
J Gen Virol. 1988 Aug;69 ( Pt 8):1841-5. doi: 10.1099/0022-1317-69-8-1841.
Exudation of polymorphonuclear leukocytes (PMN) from the infected mucosa is a characteristic feature of influenza virus infection. Since reactive oxygen species generated by PMN can be strong mutagens, the possibility of production of antigenic variants of the virus by virus-PMN interaction was investigated. Cloned influenza A NWS (H1N1) virus multiplying in Madin-Darby canine kidney cells was treated with human peripheral PMN. Assays in the presence and absence of monoclonal antibody to the cloned virus showed a seven- to ten-fold increase in the frequency of variants in the presence of PMN. The mutagenic effect was abolished by addition of superoxide dismutase to the culture.
多形核白细胞（PMN）从受感染的黏膜渗出是流感病毒感染的一个特征。由于PMN产生的活性氧可成为强诱变剂，因此研究了病毒与PMN相互作用产生病毒抗原变体的可能性。在感染了犬肾传代细胞（Madin-Darby canine kidney cells）的情况下，对克隆的甲型流感病毒NWS（H1N1）进行繁殖，并用人类外周血PMN进行处理。在有和没有针对该克隆病毒的单克隆抗体的情况下进行检测，结果显示在存在PMN的情况下，变体频率增加了7至10倍。通过向培养物中添加超氧化物歧化酶，诱变作用被消除。
