编码腮腺炎病毒P蛋白的基因的分子克隆及序列分析：腮腺炎病毒P基因是单顺反子的。
Molecular cloning and sequence analysis of the mumps virus gene encoding the P protein: mumps virus P gene is monocistronic.
作者信息
Takeuchi K, Hishiyama M, Yamada A, Sugiura A
机构信息
Department of Measles Virus, National Institute of Health, Tokyo, Japan.
出版信息
J Gen Virol. 1988 Aug;69 ( Pt 8):2043-9. doi: 10.1099/0022-1317-69-8-2043.
The nucleotide sequence of the P (phosphoprotein) gene of two strains of mumps virus has been determined from overlapping cDNA clones. The P gene contained a single open reading frame coding for a protein of 391 amino acids with a calculated Mr of 41,587, in good agreement with the value (40K to 45K) estimated from electrophoretic mobility on SDS-polyacrylamide gels. No open reading frame analogous to the C gene of other paramyxoviruses existed in the mumps virus P gene region. Comparison of the amino acid sequence of the mumps virus P protein with that of Newcastle disease virus showed a limited sequence homology.
通过重叠的互补DNA克隆，已确定了两株腮腺炎病毒P（磷蛋白）基因的核苷酸序列。P基因包含一个单一的开放阅读框，编码一种由391个氨基酸组成的蛋白质，计算所得的分子量为41587，与通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳迁移率估算的值（40K至45K）高度一致。在腮腺炎病毒P基因区域不存在与其他副粘病毒C基因类似的开放阅读框。将腮腺炎病毒P蛋白的氨基酸序列与新城疫病毒的氨基酸序列进行比较，结果显示序列同源性有限。
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