Altincekic Nadide, Korn Sophie Marianne, Qureshi Nusrat Shahin, Dujardin Marie, Ninot-Pedrosa Martí, Abele Rupert, Abi Saad Marie Jose, Alfano Caterina, Almeida Fabio C L, Alshamleh Islam, de Amorim Gisele Cardoso, Anderson Thomas K, Anobom Cristiane D, Anorma Chelsea, Bains Jasleen Kaur, Bax Adriaan, Blackledge Martin, Blechar Julius, Böckmann Anja, Brigandat Louis, Bula Anna, Bütikofer Matthias, Camacho-Zarco Aldo R, Carlomagno Teresa, Caruso Icaro Putinhon, Ceylan Betül, Chaikuad Apirat, Chu Feixia, Cole Laura, Crosby Marquise G, de Jesus Vanessa, Dhamotharan Karthikeyan, Felli Isabella C, Ferner Jan, Fleischmann Yanick, Fogeron Marie-Laure, Fourkiotis Nikolaos K, Fuks Christin, Fürtig Boris, Gallo Angelo, Gande Santosh L, Gerez Juan Atilio, Ghosh Dhiman, Gomes-Neto Francisco, Gorbatyuk Oksana, Guseva Serafima, Hacker Carolin, Häfner Sabine, Hao Bing, Hargittay Bruno, Henzler-Wildman K, Hoch Jeffrey C, Hohmann Katharina F, Hutchison Marie T, Jaudzems Kristaps, Jović Katarina, Kaderli Janina, Kalniņš Gints, Kaņepe Iveta, Kirchdoerfer Robert N, Kirkpatrick John, Knapp Stefan, Krishnathas Robin, Kutz Felicitas, Zur Lage Susanne, Lambertz Roderick, Lang Andras, Laurents Douglas, Lecoq Lauriane, Linhard Verena, Löhr Frank, Malki Anas, Bessa Luiza Mamigonian, Martin Rachel W, Matzel Tobias, Maurin Damien, McNutt Seth W, Mebus-Antunes Nathane Cunha, Meier Beat H, Meiser Nathalie, Mompeán Miguel, Monaca Elisa, Montserret Roland, Mariño Perez Laura, Moser Celine, Muhle-Goll Claudia, Neves-Martins Thais Cristtina, Ni Xiamonin, Norton-Baker Brenna, Pierattelli Roberta, Pontoriero Letizia, Pustovalova Yulia, Ohlenschläger Oliver, Orts Julien, Da Poian Andrea T, Pyper Dennis J, Richter Christian, Riek Roland, Rienstra Chad M, Robertson Angus, Pinheiro Anderson S, Sabbatella Raffaele, Salvi Nicola, Saxena Krishna, Schulte Linda, Schiavina Marco, Schwalbe Harald, Silber Mara, Almeida Marcius da Silva, Sprague-Piercy Marc A, Spyroulias Georgios A, Sreeramulu Sridhar, Tants Jan-Niklas, Tārs Kaspars, Torres Felix, Töws Sabrina, Treviño Miguel Á, Trucks Sven, Tsika Aikaterini C, Varga Krisztina, Wang Ying, Weber Marco E, Weigand Julia E, Wiedemann Christoph, Wirmer-Bartoschek Julia, Wirtz Martin Maria Alexandra, Zehnder Johannes, Hengesbach Martin, Schlundt Andreas
Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.
Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany.
Front Mol Biosci. 2021 May 10;8:653148. doi: 10.3389/fmolb.2021.653148. eCollection 2021.
The highly infectious disease COVID-19 caused by the SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium's collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on , we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form.
由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起的高传染性疾病COVID-19对人类构成了严重威胁，需要将科学力量和标准重新导向有组织的研究项目。国际联盟旨在通过汇聚全球科学专业知识来提供此类新方法。特别是，提供病毒蛋白和RNA将为详细了解SARS-CoV-2分子成分铺平道路。联盟内部的研究以及通过联盟提供的资源已完全公开，以加速获取和利用。对病毒分子成分的核磁共振（NMR）研究旨在为进一步的工作提供必要基础，包括大分子相互作用研究和高通量药物筛选。在此，我们展示了基于联盟共同努力的全面SARS-CoV-2蛋白制备方法的详尽目录。我们提供了用于大规模生产所有SARS-CoV-2蛋白或其重要部分中80%以上的方案。其中几种蛋白在多个实验室中生产，证明了全球NMR团队之间的高度互操作性。对于大多数蛋白，我们能够生产HSQC级的同位素标记样品。连同在[具体网站]上公开的几个NMR化学位移归属，我们在此为以同位素标记形式生产SARS-CoV-2蛋白提供了极具价值的资源。
