Caviedes-Bucheli J, Gutierrez-Guerra J E, Salazar F, Pichardo D, Moreno G C, Munoz H R
Graduate Studies Department, School of Dentistry, Pontificia Universidad Jeveriana, Bogota, Colombia.
Int Endod J. 2007 Feb;40(2):106-11. doi: 10.1111/j.1365-2591.2006.01189.x.
To use radioreceptor analysis for comparing substance P (SP) receptor expression in human pulp tissue samples collected from teeth having a clinical diagnosis of acute irreversible pulpitis, healthy pulps and teeth with induced inflammation.
Five pulp samples were obtained from teeth having a clinical diagnosis of acute irreversible pulpitis. Another 10 pulp samples were obtained from healthy premolars where extraction was indicated for orthodontic purposes. In five of these premolars inflammation was induced prior to pulp collection. All of the samples were processed and labelled with 125I-SP. Binding sites were identified by 125I-SP and standard SP competition assays. Kruskal-Wallis and Mann-Whitney (post-hoc) tests were used to establish statistically significant differences between the groups.
Substance P receptor expression was found in all human pulp tissue samples. Most receptors were found in the group of pulps from teeth having a clinical diagnosis of acute irreversible pulpitis, followed by the group of pulps having induced inflammation. The least number of receptors was expressed in the group of healthy pulps. Statistical analysis revealed significant differences between the group of healthy pulp and both inflamed pulp groups (P < 0.01).
Substance P receptor expression in human pulp tissue is significantly increased during inflammatory phenomena such as acute irreversible pulpitis.
运用放射受体分析法比较从临床诊断为急性不可逆性牙髓炎的牙齿、健康牙髓以及诱导炎症的牙齿中采集的人牙髓组织样本中P物质（SP）受体的表达情况。
从临床诊断为急性不可逆性牙髓炎的牙齿中获取5份牙髓样本。另外从因正畸需要而拔除的健康前磨牙中获取10份牙髓样本。在其中5颗前磨牙的牙髓采集前诱导炎症。所有样本均进行处理并用125I-SP标记。通过125I-SP和标准SP竞争试验鉴定结合位点。采用Kruskal-Wallis和Mann-Whitney（事后检验）检验来确定各组之间的统计学显著差异。
在所有人牙髓组织样本中均发现了P物质受体表达。在临床诊断为急性不可逆性牙髓炎的牙齿的牙髓组中发现的受体最多，其次是诱导炎症的牙髓组。健康牙髓组中表达的受体数量最少。统计分析显示健康牙髓组与两个炎症牙髓组之间存在显著差异（P < 0.01）。
在诸如急性不可逆性牙髓炎等炎症现象期间，人牙髓组织中P物质受体的表达显著增加。
