Impact of Albendazole on Cytokine and Chemokine Response Profiles in -Inoculated Mice.

OBJECTIVE

Alveolar echinococcosis (AE) is a zoonosis caused by the larval stage of the metacestode with a tumor-like behavior in the targeted organ, especially in the liver. Surgery with albendazole is first-line modality for AE. Drug discontinuation is usually based upon the parasitic viability shown by the positron emission tomography (PET) scan. However, as a demanding and expensive method, it is not widely practiced in majority of the endemic regions. Further understanding on the cytokine and chemokine response profiles in AE patients may provide an interesting insight for potential markers in viability assessment.

METHODS

Mice were inoculated with intrahepatically to develop the hepatic AE murine model. Oral albendazole administration was then applied for three months after the first inoculation, and peripheral and regional immune cells including type 1 T helper cells (Th), Th2, Th17, regulatory T (Treg) cells, related cytokines, and chemokines were examined.

RESULTS

The hepatic AE lesion was confirmed by ultrasound examination resulting in a successful rate of 70%. Among the 17 cytokines and chemokines detected, plasma levels of IL-23 were significantly higher in -infected mice when compared to the control group; furthermore, more obvious increasing levels were found after albendazole treatment ( < 0.05). All chemokine levels other than eotaxin and MCP-3 were slightly higher in -infected mice compared to the control group ( > 0.05). Eotaxin levels were significantly decreased in mice with infection followed by albendazole treatment ( < 0.05). Both IL-17A and IL-23 expressions in hepatic AE lesions were significantly higher and related with disease activity.

CONCLUSION

Albendazole administration influenced the balance of immune response and promotes the secretion of proinflammatory factors which is beneficial to parasite clearance. IL-23 seems to be associated with the successful albendazole treatment in mice with infection; such a change could be translated into clinical application in the near future.